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Literature summary extracted from
- Purification and characterization of Erwinia chrysanthemi B374 pectin methylesterase produced by Bacillus subtilis (1992), Enzyme Microb. Technol., 14, 832-836.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.11 | repeated freezing and thawing results in a substantial loss of activity | Dickeya chrysanthemi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.11 | D-galacturonate | slight | Dickeya chrysanthemi |  |
| 3.1.1.11 | Polygalacturonate | competitive | Dickeya chrysanthemi | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.11 | Dickeya chrysanthemi | - |
- |
- |
| 3.1.1.11 | Dickeya chrysanthemi B374 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.11 | - |
Dickeya chrysanthemi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 1.19 | - |
- |
Dickeya chrysanthemi |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.11 | 4°C, stable for at least 1 week | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.11 | anhydrogalacturonate + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| 3.1.1.11 | anhydrogalacturonate + H2O | - |
Dickeya chrysanthemi B374 | ? | - |
? | |
| 3.1.1.11 | pectin + H2O | - |
Dickeya chrysanthemi | methanol + pectate | - |
? | |
| 3.1.1.11 | pectin + H2O | - |
Dickeya chrysanthemi B374 | methanol + pectate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 50 | - |
- |
Dickeya chrysanthemi |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | - |
65 | 0°C: about 20% of maximal activity, 65°C: 10% of maximal activity | Dickeya chrysanthemi |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 40 | - |
16 min, in absence of substrate, stable up to | Dickeya chrysanthemi |
| 3.1.1.11 | 50 | - |
pH 6.0, 16 min, 50% loss of activity | Dickeya chrysanthemi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 5 | - |
- |
Dickeya chrysanthemi |
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Release 2023.1 (January 2023)
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