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Literature summary extracted from

  • Lim, J.Y.; Fujio, Y.; Ueda, S.
    Purification and characterization of pectinesterase and pectin lyase from Aspergillus oryzae A-3 (1983), J. Appl. Biochem., 5, 91-98.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.10 iodoacetate 10 mM, stimulates Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 Cu2+
-
Aspergillus oryzae
3.1.1.11 Hg2+
-
Aspergillus oryzae
3.1.1.11 iodoacetic acid
-
Aspergillus oryzae
3.1.1.11 NEM
-
Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.10 additional information
-
additional information Km for pectin: 1.36 mg/ml Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.11 Mg2+ stimulates Aspergillus oryzae
3.1.1.11 NaCl enzyme is not affected by NaCl from 0.1 M to 0.5 M concentrations Aspergillus oryzae
4.2.2.10 Ca2+ stimulates Aspergillus oryzae
4.2.2.10 Cu2+ stimulates Aspergillus oryzae
4.2.2.10 Hg2+ stimulates Aspergillus oryzae
4.2.2.10 Mg2+ stimulates Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 34000
-
gel filtration Aspergillus oryzae
4.2.2.10 34000
-
gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus oryzae
-
A-3
-
3.1.1.11 Aspergillus oryzae A-3
-
A-3
-
4.2.2.10 Aspergillus oryzae
-
A-3
-
4.2.2.10 Aspergillus oryzae A-3
-
A-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 isoenzyme PE I and PE II Aspergillus oryzae
3.1.1.11 isoenzyme PE II Aspergillus oryzae
4.2.2.10
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.11 additional information
-
-
Aspergillus oryzae
4.2.2.10 additional information
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
Aspergillus oryzae methanol + pectate
-
?
3.1.1.11 pectin + H2O
-
Aspergillus oryzae A-3 methanol + pectate
-
?
4.2.2.10 pectin
-
Aspergillus oryzae ?
-
?
4.2.2.10 pectin
-
Aspergillus oryzae A-3 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 50 55 isoenzyme PE I and PE II Aspergillus oryzae
4.2.2.10 50 55
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 40
-
30 min, pH 4.0-9.0, isoenzyme PE II is stable Aspergillus oryzae
3.1.1.11 50
-
pH 8.0, stable up to Aspergillus oryzae
4.2.2.10 40
-
30 min, pH 4.0-8.5., stable Aspergillus oryzae
4.2.2.10 50
-
pH 8.0, 30 min, stable up to Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 8.5
-
-
Aspergillus oryzae
4.2.2.10 8.5
-
-
Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.11 4 9 40°C, 30 min, stable Aspergillus oryzae
4.2.2.10 4 8.5 30 min, 40°C, stable Aspergillus oryzae