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Literature summary extracted from

  • Huang, C.S.; Moore, W.R.; Meister, A.
    On the active site thiol of gamma-glutamylcysteine synthetase: relationship to catalysis, inhibition, and regulation (1988), Proc. Natl. Acad. Sci. USA, 85, 2464-2468.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 4-Methylene glutamate no inhibition Escherichia coli
6.3.2.2 4-Methylene glutamate
-
 Rattus norvegicus
6.3.2.2 buthionine sulfoximine only in presence of ATP Escherichia coli
6.3.2.2 buthionine sulfoximine
-
 Rattus norvegicus
6.3.2.2 cystamine no inhibition Escherichia coli
6.3.2.2 cystamine
-
 Rattus norvegicus
6.3.2.2 glutathione feed-back inhibition Escherichia coli
6.3.2.2 glutathione feed-back inhibition Rattus norvegicus
6.3.2.2 S-sulfocysteine no inhibition Escherichia coli
6.3.2.2 S-sulfocysteine
-
 Rattus norvegicus
6.3.2.2 S-sulfohomocysteine no inhibition Escherichia coli
6.3.2.2 S-sulfohomocysteine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.1
-
 L-Cys strain W Escherichia coli
6.3.2.2 0.2
-
 L-Cys
-
 Rattus norvegicus
6.3.2.2 0.2
-
 L-Cys strain KM Escherichia coli
6.3.2.2 0.7
-
 L-Glu strain W Escherichia coli
6.3.2.2 1
-
 L-2-aminobutanoate
-
 Rattus norvegicus
6.3.2.2 1.3
-
 L-2-aminobutanoate
-
 Rattus norvegicus
6.3.2.2 1.3
-
 L-2-aminobutanoate strain W Escherichia coli
6.3.2.2 1.4
-
 L-2-aminobutanoate
-
 Rattus norvegicus
6.3.2.2 1.4
-
 L-2-aminobutanoate strain KM Escherichia coli
6.3.2.2 1.7
-
 L-Glu strain KM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 56000
-
 gel filtration, native PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Escherichia coli
-
 enriched by recombinant DNA techniques in its content of gamma-glutamylcysteine synthetase, strain W, strain KM
-
6.3.2.2 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 31
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + beta-chloro-L-Ala
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
-
 ?
6.3.2.2 ATP + L-Glu + beta-chloro-L-Ala strain KM: 79% of the activity relative to L-Cys, strain W: 99% of the activity relative to L-Cys Escherichia coli ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
-
 ?
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate strain KM: 85% of the activity relative to L-Cys, strain W: 81% of the activity relative to L-Cys Escherichia coli ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Ser strain KM: 18% of the activity relative to L-Cys, strain W: 13% of the activity relative to L-Cys Escherichia coli ADP + phosphate + gamma-L-Glu-L-Ser
-
 ?
6.3.2.2 ATP + L-Glu + S-methyl-L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-S-methyl-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + S-methyl-L-Cys strain KM: 70% of the activity relative to L-Cys, strain W: 70% of the activity relative to L-Cys Escherichia coli ADP + phosphate + gamma-L-Glu-S-methyl-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 monomer 1 * 56000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.2 additional information
-
 additional information
-
 Escherichia coli
6.3.2.2 additional information
-
 additional information
-
 Rattus norvegicus