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Literature summary extracted from

  • Kim, S.T.; Huh, W.K.; Kim, J.Y.; Hwang, S.W.; Kang, S.O.
    D-Arabinose dehydrogenase and biosynthesis of erythroascorbic acid in Candida albicans (1996), Biochim. Biophys. Acta, 1297, 1-8.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.37 repeated freezing at -20°C and thawing results in rapid and irreversible loss of activity Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.116 Cu2+
-
 Candida albicans
1.1.1.116 Fe2+
-
 Candida albicans
1.1.1.116 Hg2+
-
 Candida albicans
1.1.1.116 Mg2+
-
 Candida albicans
1.1.1.116 Mn2+
-
 Candida albicans
1.1.1.116 N-ethylmaleimide
-
 Candida albicans
1.1.1.116 NADPH
-
 Candida albicans
1.1.1.116 p-chloromercuribenzoate
-
 Candida albicans
1.1.1.116 Zn2+
-
 Candida albicans
1.1.1.117 Cu2+
-
 Candida albicans
1.1.1.117 Fe2+
-
 Candida albicans
1.1.1.117 Hg2+
-
 Candida albicans
1.1.1.117 Mg2+
-
 Candida albicans
1.1.1.117 Mn2+
-
 Candida albicans
1.1.1.117 NADPH competitive to NADP+ Candida albicans
1.1.1.117 Zn2+
-
 Candida albicans
1.1.3.37 Cd2+
-
 Candida albicans
1.1.3.37 D-galactono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
1.1.3.37 D-glucono-1,5-lactone competitive to D-arabinono-1,4-lactone Candida albicans
1.1.3.37 D-gulono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
1.1.3.37 Hg2+
-
 Candida albicans
1.1.3.37 iodoacetamide
-
 Candida albicans
1.1.3.37 iodoacetic acid
-
 Candida albicans
1.1.3.37 L-arabinono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
1.1.3.37 Mn2+
-
 Candida albicans
1.1.3.37 NEM
-
 Candida albicans
1.1.3.37 PCMB
-
 Candida albicans
1.1.3.37 Zn2+
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.116 28.2
-
 L-fucose
-
 Candida albicans
1.1.1.116 29.2
-
 D-arabinose
-
 Candida albicans
1.1.1.116 37.1
-
 L-Xylose
-
 Candida albicans
1.1.1.116 91.3
-
 L-galactose
-
 Candida albicans
1.1.1.117 0.0446
-
 NADP+
-
 Candida albicans
1.1.1.117 28.9
-
 L-fucose
-
 Candida albicans
1.1.1.117 29.9
-
 D-arabinose
-
 Candida albicans
1.1.1.117 37.1
-
 L-Xylose
-
 Candida albicans
1.1.1.117 60
-
 D-arabinose
-
 Candida albicans
1.1.1.117 91.3
-
 L-galactose
-
 Candida albicans
1.1.3.37 44.1
-
 D-arabinono-1,4-lactone
-
 Candida albicans
1.1.3.37 53.7
-
 L-galactono-1,4-lactone
-
 Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.117 cytoplasm
-
 Candida albicans 5737
-
1.1.3.37 mitochondrion
-
 Candida albicans 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.116 41000
-
 gel filtration Candida albicans
1.1.1.116 42000
-
 1 * 42000, SDS-PAGE Candida albicans
1.1.1.117 41000
-
 gel filtration Candida albicans
1.1.1.117 42000
-
 1 * 42000, SDS-PAGE Candida albicans
1.1.3.37 66700
-
 1 * 66700, SDS-PAGE Candida albicans
1.1.3.37 84400
-
 PAGE in presence of deoxycholate Candida albicans
1.1.3.37 110000
-
 gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+ Candida albicans
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.3.37 D-arabinono-1,4-lactone + O2 Candida albicans final step in biosynthesis of D-erythroascorbic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.116 Candida albicans
-
-
-
1.1.1.117 Candida albicans
-
-
-
1.1.3.37 Candida albicans
-
 ATCC 10231
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.116
-
 Candida albicans
1.1.1.117
-
 Candida albicans
1.1.3.37
-
 Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.116 2.2
-
-
 Candida albicans
1.1.3.37 18.8
-
-
 Candida albicans

Storage Stability

EC Number Storage Stability Organism
1.1.3.37 4°C, less than 50% loss of activity after 2 months Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.116 D-arabinose + NAD+
-
 Candida albicans D-arabinono-1,4-lactone + NADH
-
 ir
1.1.1.116 D-arabinose + NADP+
-
 Candida albicans D-arabinono-1,4-lactone + NADPH
-
 ?
1.1.1.116 L-fucose + NAD+ with NADP+ Candida albicans L-fuconolactone + NADH
-
 ?
1.1.1.116 L-galactose + NADP+
-
 Candida albicans L-galactono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.116 L-xylose + NADP+
-
 Candida albicans L-xylono-1,4-lactone + NADPH
-
 ?
1.1.1.117 D-arabinose + NAD(P)+
-
 Candida albicans D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 L-fucose + NADP+
-
 Candida albicans 6-deoxy-L-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.117 L-galactose + NADP+
-
 Candida albicans L-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.117 L-xylose + NADP+
-
 Candida albicans L-xylono-1,4-lactone + NADPH
-
 ?
1.1.3.37 D-Arabinono-1,4-lactone + O2
-
 Candida albicans D-erythro-ascorbate + ?
-
 ?
1.1.3.37 D-arabinono-1,4-lactone + O2 final step in biosynthesis of D-erythroascorbic acid Candida albicans ?
-
 ?
1.1.3.37 L-Galactono-1,4-lactone + O2 87.6% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?
1.1.3.37 L-Gulono-1,4-lactone + O2 24.7% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?
1.1.3.37 L-Xylono-1,4-lactone + O2 84.6% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.116 monomer 1 * 42000, SDS-PAGE Candida albicans
1.1.1.117 monomer 1 * 42000, SDS-PAGE Candida albicans
1.1.3.37 monomer 1 * 66700, SDS-PAGE Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.116 30
-
 assay at Candida albicans
1.1.1.117 30
-
-
 Candida albicans
1.1.3.37 40
-
-
 Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.37 50
-
 above, 1 min, complete loss of activity Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.37 18.3
-
 L-galactono-1,4-lactone
-
 Candida albicans
1.1.3.37 20.9
-
 D-arabinono-1,4-lactone
-
 Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.116 8
-
-
 Candida albicans
1.1.1.117 8
-
-
 Candida albicans
1.1.3.37 6.1
-
-
 Candida albicans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.37 5 8 pH 5.0: about 20% of maximal activity, pH 8.0: about 40% of maximal activity Candida albicans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.116 4.5 7.5
-
 Candida albicans
1.1.3.37 7.5 10 4°C, 48 h, stable Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.116 NADP+
-
 Candida albicans
1.1.1.117 NADP+
-
 Candida albicans
1.1.3.37 flavin flavoprotein, the flavin may be covalently bound to the enzyme Candida albicans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.117 0.0786
-
 NADPH
-
 Candida albicans