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Literature summary extracted from

  • Fujiwara, S.; Lee, S.; Haruki, M.; Kanaya, S.; Takagi, M.; Imanaka, T.
    Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1 (1996), FEBS Lett., 394, 66-70.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.12 putrescine enhances activity, optimum concentrations is 0.07 mM Thermococcus kodakarensis
6.1.1.12 spermidine enhances activity, optimum concentrations is 0.07 mM Thermococcus kodakarensis
6.1.1.12 spermine enhances activity, optimum concentrations is 0.005 mM Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 expression in Escherichia coli Thermococcus kodakarensis
6.1.1.12 overexpression in Escherichia coli Pyrococcus sp.

General Stability

EC Number General Stability Organism
6.1.1.12 polyamines stabilize the conformation of the tRNA-AspRS complex Pyrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.12 50000
-
 2 * 50000, SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Homo sapiens
-
 wild-type and N-terminal 32-residue truncated form (hDRS delta 32) expressed in Escherichia coli
-
6.1.1.12 Pyrococcus sp.
-
 overexpressed in Escherichia coli
-
6.1.1.12 Pyrococcus sp. KOD1
-
 overexpressed in Escherichia coli
-
6.1.1.12 Thermococcus kodakarensis Q52428
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12
-
 Pyrococcus sp.
6.1.1.12
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Homo sapiens AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 GTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 GTP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 GTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 UTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 UTP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 UTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.12 dimer 2 * 50000, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 ASPA
-
 Thermococcus kodakarensis
6.1.1.12 Aspartyl-tRNA synthetase
-
 Thermococcus kodakarensis
6.1.1.12 AspRS
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.12 65
-
-
 Pyrococcus sp.
6.1.1.12 65
-
 optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine Thermococcus kodakarensis
6.1.1.12 75
-
 in presence of polyamine molecules, such as putrescine, spermine, and spermidine Pyrococcus sp.
6.1.1.12 75
-
 optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.12 35 75 35°C: about 60% of maximal activity, 75°C: about 50% of maximal activity Thermococcus kodakarensis
6.1.1.12 45 85 45°C: about 80% of maximal activity, 85°C: about 25% of maximal activity Pyrococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.12 70
-
 no denaturation below Pyrococcus sp.
6.1.1.12 85
-
 the thermal melting temperature seems higher than 85°C in the presence of Mg2+. In the absence of Mg 2+, melting temperature decreases to 85°C Thermococcus kodakarensis
6.1.1.12 90
-
 half-life is 17 h Pyrococcus sp.
6.1.1.12 90
-
 half-life: 17 h Thermococcus kodakarensis
6.1.1.12 100
-
 half-life is 3.5 h Pyrococcus sp.
6.1.1.12 100
-
 half-life: 3.5 h Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.12 7.5
-
-
 Pyrococcus sp.
6.1.1.12 7.5
-
-
 Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.12 5.5 9 pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity Pyrococcus sp.
6.1.1.12 5.5 8 pH 5.5: about 60% of maximal activity, pH 8.0: about 65% of maximal activity Thermococcus kodakarensis