Literature summary extracted from

  • Reusch, V.M.; Neuhaus, F.C.
    D-Alanine:membrane acceptor ligase from Lactobacillus casei (1971), J. Biol. Chem., 246, 6136-6143.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.13 Cu2+
-
Lacticaseibacillus casei
6.1.1.13 D-2-Amino-n-butyric acid hydroxamate
-
Lacticaseibacillus casei
6.1.1.13 DL-2-Amino-n-butyric acid hydroxamate
-
Lacticaseibacillus casei
6.1.1.13 DL-Alanine amide
-
Lacticaseibacillus casei
6.1.1.13 DL-Alanine hydroxamate
-
Lacticaseibacillus casei
6.1.1.13 D-Ser
-
Lacticaseibacillus casei
6.1.1.13 KCl
-
Lacticaseibacillus casei
6.1.1.13 additional information not inhibitory are D-Ala, L-Ala, L-2-amino-n-butyric acid, Gly, D-Thr, D-Ile, L-Ile, D-cycloserine, L-cycloserine, DL-alaniol Lacticaseibacillus casei
6.1.1.13 NaCl
-
Lacticaseibacillus casei
6.1.1.13 Ni2+
-
Lacticaseibacillus casei
6.3.2.16 Cu2+
-
Lacticaseibacillus casei
6.3.2.16 D-2-amino-n-butanoate
-
Lacticaseibacillus casei
6.3.2.16 DL-2-Amino-n-butanoyl hydroxamate
-
Lacticaseibacillus casei
6.3.2.16 DL-Ala amide
-
Lacticaseibacillus casei
6.3.2.16 DL-Alanine hydroxamate
-
Lacticaseibacillus casei
6.3.2.16 D-Ser
-
Lacticaseibacillus casei
6.3.2.16 Ni2+
-
Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.16 0.018
-
D-Ala
-
Lacticaseibacillus casei
6.1.1.13 0.018
-
D-Ala
-
Lacticaseibacillus casei
6.3.2.16 3.3
-
ATP
-
Lacticaseibacillus casei
6.1.1.13 3.3
-
ATP
-
Lacticaseibacillus casei
6.3.2.16 0.85
-
D-2-amino-n-butanoate
-
Lacticaseibacillus casei
6.1.1.13 0.85
-
D-2-amino-n-butyric acid
-
Lacticaseibacillus casei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.13 membrane
-
Lacticaseibacillus casei
-
-
6.1.1.13 soluble
-
Lacticaseibacillus casei
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.16 Mg2+ stimulates Lacticaseibacillus casei
6.3.2.16 Mn2+ stimulates Lacticaseibacillus casei
6.3.2.16 Ca2+ stimulates Lacticaseibacillus casei
6.3.2.16 Co2+ stimulates Lacticaseibacillus casei
6.3.2.16 Ba2+ stimulates Lacticaseibacillus casei
6.1.1.13 Mg2+ enhancement of activity, Mg2+ can be replaced by Ba2+, Ca2+, Co2+, K+, Na+ Lacticaseibacillus casei
6.1.1.13 Ba2+ Ba2+ can replace Mg2+ in activity enhancement Lacticaseibacillus casei
6.1.1.13 Ca2+ Ca2+ can replace Mg2+ in activity enhancement Lacticaseibacillus casei
6.1.1.13 Co2+ Co2+ can replace Mg2+ in activity enhancement Lacticaseibacillus casei
6.1.1.13 K+ K+ can replace Mg2+ in activity enhancement Lacticaseibacillus casei
6.1.1.13 Na+ Na+ can replace Mg2+ in activity enhancement Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.16 58000
-
gel filtration Lacticaseibacillus casei
6.1.1.13 39000
-
gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.16 ATP + D-Ala + membrane-acceptor Lacticaseibacillus casei reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids ?
-
?
6.1.1.13 D-Alanine + ATP + ? Lacticaseibacillus casei biosynthesis of teichoic acid ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.16 Lacticaseibacillus casei
-
-
-
6.1.1.13 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.13 partial Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.16 ATP + D-Ala + membrane-acceptor no activity with L-Ala Lacticaseibacillus casei ADP + phosphate + D-alanyl-membrane acceptor
-
?
6.3.2.16 ADP + D-Ala + membrane-acceptor 83% of the activity relative to ATP Lacticaseibacillus casei AMP + phosphate + D-alanyl-membrane acceptor
-
?
6.3.2.16 GTP + D-Ala + membrane-acceptor 84% of the activity relative to ATP Lacticaseibacillus casei GDP + phosphate + D-alanyl-membrane acceptor
-
?
6.3.2.16 CTP + D-Ala + membrane-acceptor 107% of the activity relative to ATP Lacticaseibacillus casei CDP + phosphate + D-alanyl-membrane acceptor
-
?
6.3.2.16 UTP + D-Ala + membrane-membrane acceptor 126% of the activity relative to ATP Lacticaseibacillus casei UDP + phosphate + D-alanyl-membrane acceptor
-
?
6.3.2.16 ATP + D-2-amino-n-butanoate + membrane-acceptor
-
Lacticaseibacillus casei ADP + phosphate + D-2-aminobutanoyl-membrane acceptor
-
?
6.1.1.13 ATP + D-Ala + membrane fragments ATP can be substituted by ADP, GTP, CTP, UTP, not by AMP, D-Ala cannot be substituted by L-Ala Lacticaseibacillus casei D-Alanyl-membrane fragments + AMP + diphosphate
-
?
6.1.1.13 ATP + D-2-amino-n-butyric acid + membrane fragments not L-2-amino-n-butyric acid Lacticaseibacillus casei D-2-Amino-n-butryl-membrane fragments + AMP + diphosphate
-
?
6.3.2.16 ATP + D-Ala + membrane-acceptor reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids Lacticaseibacillus casei ?
-
?
6.1.1.13 D-Alanine + ATP + ? biosynthesis of teichoic acid Lacticaseibacillus casei ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.16 6.5 7
-
Lacticaseibacillus casei
6.1.1.13 6.5 7
-
Lacticaseibacillus casei