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Literature summary extracted from

  • Agou, F.; Waller, J.P.; Mirande, M.
    Expression of rat aspartyl-tRNA synthetase in Saccharomyces cerevisiae. Role of the NH2-terminal polypeptide extension on enzyme activity and stability (1996), J. Biol. Chem., 271, 29295-29303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 expression in Saccharomyces cerevisiae, wild-type enzyme and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.12 additional information two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, expressed in Saccharomyces cerevisiae, are less stable than wild-type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.12 0.0005
-
 tRNAAsp aspartyl-tRNA synthetase as a component of the multisynthetase complex or recombinant native enzyme Rattus norvegicus
6.1.1.12 0.0006
-
 tRNAAsp tRNAAsp, , truncated enzymes AspRS-DELTAN36 and AspRS-DELTAN20 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Mammalia
-
-
-
6.1.1.12 Rattus norvegicus
-
 wild-type and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, overexpressed in Saccharomyces cerevisiae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 wild-type and two truncated forms Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.12 0.395
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Mammalia AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Rattus norvegicus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.12 additional information enzyme occurs in the multienzyme complex of aminoacyl-tRNA synthetases Mammalia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.12 additional information
-
 additional information
-
 Rattus norvegicus