Any feedback?
Literature summary extracted from
- Purification and properties of the dihydrofolate synthetase from pea seedlings (1975), J. Nutr. Sci. Vitaminol., 21, 7-18.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.3.2.12 | 0.001 | - |
7,8-dihydropteroate | - |
Pisum sativum |  |
| 6.3.2.12 | 0.1 | - |
ATP | - |
Pisum sativum | |
| 6.3.2.12 | 1.5 | - |
Glu | - |
Pisum sativum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.2.12 | K+ | K+ is most effective | Pisum sativum | |
| 6.3.2.12 | K+ | monovalent cation requirement is satisfied by K+, Rb+ or NH4+ | Pisum sativum | |
| 6.3.2.12 | Mg2+ | Km: 1.1 mM | Pisum sativum | |
| 6.3.2.12 | Mn2+ | Km: 0.063 mM | Pisum sativum | |
| 6.3.2.12 | Mn2+ | divalent cation required | Pisum sativum | |
| 6.3.2.12 | NH4+ | - |
Pisum sativum | |
| 6.3.2.12 | Rb+ | monovalent cation requirement is satisfied by K+, Rb+ or NH4+ | Pisum sativum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.12 | 56000 | - |
gel filtration | Pisum sativum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.2.12 | Pisum sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.2.12 | - |
Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.3.2.12 | seedling | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.12 | 0.007 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.2.12 | ATP + 7,8-dihydropteroate + L-Glu | - |
Pisum sativum | ADP + phosphate + 7,8-dihydrofolate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.12 | 8.8 | - |
- |
Pisum sativum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.2.12 | 7.5 | 11 | 7.5: about 50% of maximal activity, 11.0: about 80% of activity maximum | Pisum sativum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
