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Literature summary extracted from

  • Escalante, C.; Yang, D.C.
    Expression of human aspartyl-tRNA synthetase in Escherichia coli. Functional analysis of the N-terminal putative amphiphilic helix (1993), J. Biol. Chem., 268, 6014-6023.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 wild-type enzyme and N-terminal 32-residues truncated form, expressed in Escherichia coli as fusion proteins linked through a thrombin cleavage site with glutathione-S-transferase Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.12 hDRSDELTA32 N-terminal 32-residue truncated form, hDRSDELTA32, with lower thermal stability and ATP-diphosphate exchange activity, but higher aminoacylation activity. Fusion protein of glutathione-S-transferase and hDRSDELTA32 with lower thermal stability Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.12 0.000013
-
 tRNAAsp native enzyme Homo sapiens
6.1.1.12 0.00013
-
 tRNAAsp truncated enzyme Homo sapiens
6.1.1.12 0.029
-
 Asp truncated enzyme Homo sapiens
6.1.1.12 0.302
-
 Asp native enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Bacteria
-
-
-
6.1.1.12 Homo sapiens
-
 wild-type and N-terminal 32-residue truncated form (hDRS delta 32) expressed in Escherichia coli
-
6.1.1.12 Mammalia
-
-
-
6.1.1.12 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 wild-type enzyme and N-terminal 32-residue truncated form (hDRS delta 32), expressed in Escherichia coli as fusion proteins linked through a thrombin cleavage site with glutathione-S-transferase Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.12 0.017
-
 wild-type enzyme Homo sapiens
6.1.1.12 0.034
-
 truncated enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Mammalia AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Bacteria AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Homo sapiens AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Saccharomyces cerevisiae AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.12 additional information enzyme occurs in the multienzyme complex of aminoacyl-tRNA synthetases Mammalia
6.1.1.12 additional information enzymes exist as free soluble enzymes Bacteria
6.1.1.12 additional information enzymes exist as free soluble enzymes Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.12 37
-
 native enzyme is stable for 24 h, half-life of truncated enzyme hDRSDELTA32 is 7 h, half-life of the fusion protein of glutathione-S-transferase and hDRSDELTA32 is 3 h Homo sapiens