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Literature summary extracted from

  • Bognar, A.L.; Shane, B.
    Bacterial folylpoly(gamma-glutamate) synthase-dihydrofolate synthase (1986), Methods Enzymol., 122, 349-359.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17
-
 Escherichia coli

General Stability

EC Number General Stability Organism
6.3.2.17 K+ and phosphate stabilize Corynebacterium sp.
6.3.2.17 K+ and phosphate stabilize Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.12 additional information 5,6,7,8-tetrahydrofolate does not significantly inhibit Corynebacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.12 0.0029
-
 ATP
-
 Corynebacterium sp.
6.3.2.12 1.38
-
 L-Glu
-
 Corynebacterium sp.
6.3.2.17 0.0021
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Corynebacterium sp.
6.3.2.17 0.0023
-
 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
 Lacticaseibacillus casei
6.3.2.17 0.018
-
 ATP
-
 Corynebacterium sp.
6.3.2.17 0.16
-
 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu L-Glu Corynebacterium sp.
6.3.2.17 0.423
-
 L-Glu
-
 Lacticaseibacillus casei
6.3.2.17 5.6
-
 ATP
-
 Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ required form maximal activity Escherichia coli
6.3.2.17 K+ required form maximal activity Corynebacterium sp.
6.3.2.17 K+ required form maximal activity Lacticaseibacillus casei
6.3.2.17 K+ maximal activity at 200 mM Escherichia coli
6.3.2.17 K+ maximal activity at 200 mM Corynebacterium sp.
6.3.2.17 K+ maximal activity at 200 mM Lacticaseibacillus casei
6.3.2.17 Mg2+ required Escherichia coli
6.3.2.17 Mg2+ required Corynebacterium sp.
6.3.2.17 Mg2+ required Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.12 51000
-
 gel filtration Corynebacterium sp.
6.3.2.12 53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.
6.3.2.17 43000
-
 gel filtration Lacticaseibacillus casei
6.3.2.17 51000
-
 gel filtration Corynebacterium sp.
6.3.2.17 53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Corynebacterium sp.
-
 enzyme posseses both dihydrofolate synthetase activity and folylpolyglutamate synthetase activity
-
6.3.2.17 Corynebacterium sp.
-
-
-
6.3.2.17 Escherichia coli
-
-
-
6.3.2.17 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.12
-
 Corynebacterium sp.
6.3.2.17
-
 Corynebacterium sp.
6.3.2.17
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.12 additional information
-
-
 Corynebacterium sp.
6.3.2.17 0.23
-
-
 Lacticaseibacillus casei
6.3.2.17 0.57
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Corynebacterium sp. ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-glutamate ATP in form of MgATP2- Corynebacterium sp. ADP + phosphate + 7,8-dihydropteroylglutamate
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
-
 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate ATP in form of MgATP2- Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 additional information enzyme also posseses dihydrofolate synthetase activity Escherichia coli ?
-
 ?
6.3.2.17 additional information enzyme also posseses dihydrofolate synthetase activity Corynebacterium sp. ?
-
 ?
6.3.2.17 additional information no dihydrofolate synthetase activity Lacticaseibacillus casei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.12 monomer 1 * 53000, SDS-PAGE Corynebacterium sp.
6.3.2.17 monomer
-
 Escherichia coli
6.3.2.17 monomer
-
 Lacticaseibacillus casei
6.3.2.17 monomer 1 * 53000, SDS-PAGE Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.5
-
-
 Corynebacterium sp.
6.3.2.17 9.5
-
-
 Lacticaseibacillus casei