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Literature summary extracted from

  • Xue, Y.; Sato, S.; Razafsky, D.; Sahu, B.; Shen, S.Q.; Potter, C.; Sandell, L.L.; Corbo, J.C.; Palczewski, K.; Maeda, A.; Hodzic, D.; Kefalov, V.J.
    The role of retinol dehydrogenase 10 in the cone visual cycle (2017), Sci. Rep., 7, 2390 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.300 additional information RDH10 enzyme knockout and overexpression in retina cell. Rdh10 mRNA levels are substantially reduced in retinas obtained from Pdgfra-Cre Rdh10flox/flox mice through a knockout in M├╝ller cells. Similarly, the expression of Rdh10 is also dramatically reduced in Six3-Cre Rdh10flox/flox retinas, demonstrating its suppression in the entire retina. In contrast, Rdh10 mRNA levels are notably increased in transgenic Rdh10+ mice. The deletion of RDH10 in cones does not affect the overall number of cone cells or their function, and cone dark adaptation in vivo is unaffected by cone-specific deletion of RDH10 Mus musculus
1.1.1.300 additional information generation of mice lacking RDH10 either in cone photoreceptors, M├╝ller cells, or the entire retina. In vivo electroretinography and transretinal recordings reveal normal cone photoresponses in all RDH10-deficient mouse lines. Notably, their cone-driven dark adaptation both in vivo and in isolated retina is unaffected, indicating that RDH10 is not required for the function of the retina visual cycle. Generation of transgenic mice expressing RDH10 ectopically in rod cells. Rod dark adaptation is unaffected by the expression of RDH10 and transgenic rods are unable to use cis-retinol for pigment regeneration. Lack of phenotype of mice lacking RDH10 in the entire retina Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+ Mus musculus
-
all-trans-retinol + NADP+
-
?
1.1.1.300 retinol + NADP+ Mus musculus
-
retinal + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Mus musculus Q8VCH7
-
-
1.1.1.315 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.300 eye
-
Mus musculus
-
1.1.1.300 Mueller cell
-
Mus musculus
-
1.1.1.300 Mueller cell the enzyme is expressed abundantly in Mueller cells Mus musculus
-
1.1.1.300 retina
-
Mus musculus
-
1.1.1.300 retina RDH10 is upregulated in rod/cone hybrid retinas Mus musculus
-
1.1.1.300 retinal cone
-
Mus musculus
-
1.1.1.315 eye
-
Mus musculus
-
1.1.1.315 retina
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+
-
Mus musculus all-trans-retinol + NADP+
-
?
1.1.1.300 retinol + NADP+
-
Mus musculus retinal + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.300 RDH10
-
Mus musculus
1.1.1.300 retinol dehydrogenase 10
-
Mus musculus
1.1.1.315 11-cis-RDH
-
Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NADP+
-
Mus musculus
1.1.1.300 NADPH
-
Mus musculus

General Information

EC Number General Information Comment Organism
1.1.1.300 malfunction mice lacking RDH10 either in cone photoreceptors, M├╝ller cells, or the entire retina show normal cone photoresponses in all RDH10-deficient mouse lines. Their cone-driven dark adaptation both in vivo and in isolated retina is unaffected, indicating that RDH10 is not required for the function of the retina visual cycle. In transgenic mice overexpressing RDH10 ectopically in rod cells, rod dark adaptation is unaffected and transgenic rods are unable to use cis-retinol for pigment regeneration Mus musculus
1.1.1.300 malfunction RDH10 is not required for the function of the retina visual cycle. Transgenic mice expressing RDH10 ectopically in rod cells show, that rod dark adaptation is unaffected by the expression of RDH10 and transgenic rods are unable to use cis-retinol for pigment regeneration. Lack of phenotype of mice lacking RDH10 in the entire retina Mus musculus
1.1.1.300 physiological function pigment regeneration is critical for the function of cone photoreceptors in bright and rapidly-changing light conditions. This process is facilitated by the recently-characterized retina visual cycle, in which M├╝ller cells recycle spent all-trans-retinol visual chromophore back to 11-cis-retinol. This 11-cis-retinol is oxidized selectively in cones to the 11-cis-retinal used for pigment regeneration. Retinol dehydrogenase 10 (RDH10) is responsible for the oxidation of 11-cis-retinol in the cone visual cycle, but RDH10 is not the dominant retina 11-cis-RDH, overview. Cone RDH10 is not required for normal cone dark adaptation Mus musculus
1.1.1.300 physiological function cone-specific 11-cis-RDH is likely to be important in regulating access to the retina visual cycle. Retinol dehydrogenase 10, RDH10 (UniProt ID Q8VCH7), is not the dominant retina 11-cis-RDH. Cone RDH10 is not required for the normal function of dark-adapted cones and for normal cone dark adaptation, as well as for the retina visual cycle Mus musculus
1.1.1.315 malfunction RDH10 is not required for the function of the retina visual cycle. Transgenic mice expressing RDH10 ectopically in rod cells show, that rod dark adaptation is unaffected by the expression of RDH10 and transgenic rods were unable to use cis-retinol for pigment regeneration Mus musculus
1.1.1.315 physiological function cone-specific 11-cis-RDH is likely to be important in regulating access to the retina visual cycle. Retinol dehydrogenase 10, RDH10 (UniProt ID Q8VCH7), is not the dominant retina 11-cis-RDH, it catalyzes the all-transretinol convertion, cf. EC 1.1.1.300 Mus musculus