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Literature summary extracted from

  • Gieldon, A.; Zurawa-Janicka, D.; Jarzab, M.; Wenta, T.; Golik, P.; Dubin, G.; Lipinska, B.; Ciarkowski, J.
    Distinct 3D architecture and dynamics of the human HtrA2(Omi) protease and its mutated variants (2016), PLoS ONE, 11, e0161526 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.108 structures of variant V226K/S306A and V325D. Upon binding of specific peptide ligand NH2-GWTMFWV-COOH, the PDZ domains open more dynamically in the wild type protease compared to the V226K mutant, whereas the movement is not observed in the V325D mutant. The movement relies on a PDZ vs. protease domain rotation which opens the protease domain-PDZ interface Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 V226K increase in activtiy compared to weidl-type Homo sapiens
3.4.21.108 V226K/S306A inactive Homo sapiens
3.4.21.108 V325D inactive Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens O43464
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