BRENDA - Enzyme Database

CYP82Y1 is N-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy

Dang, T.T.; Facchini, P.J.; J. Biol. Chem. 289, 2013-2026 (2014) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.166
Papaver somniferum
I3PLR1
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.166
flower bud
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
leaf
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
root
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
stem
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
751038
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
751038
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.14.166
CYP82Y1
-
Papaver somniferum
1.14.14.166
N-methylcanadine 1-hydroxylase
-
Papaver somniferum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.166
cytochrome P-450
heme-thiolate enzyme
Papaver somniferum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.166
cytochrome P-450
heme-thiolate enzyme
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.166
flower bud
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
leaf
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
root
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
1.14.14.166
stem
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
Papaver somniferum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
751038
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.166
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
751038
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.14.166
malfunction
suppression of CYP82Y1 using virus-induced gene silencing causes a significant reduction in the levels of noscapine, narcotoline, and a putative downstream secoberbine intermediate and also results in increased accumulation of the upstream pathway intermediates scoulerine, tetrahydrocolumbamine, canadine, and N-methylcanadine
Papaver somniferum
1.14.14.166
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver somniferum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.166
malfunction
suppression of CYP82Y1 using virus-induced gene silencing causes a significant reduction in the levels of noscapine, narcotoline, and a putative downstream secoberbine intermediate and also results in increased accumulation of the upstream pathway intermediates scoulerine, tetrahydrocolumbamine, canadine, and N-methylcanadine
Papaver somniferum
1.14.14.166
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver somniferum