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Literature summary extracted from

  • Ryu, J.Y.; Seo, J.; Park, S.; Ahn, J.H.; Chong, Y.; Sadowsky, M.J.; Hur, H.G.
    Characterization of an isoeugenol monooxygenase (Iem) from Pseudomonas nitroreducens Jin1 that transforms isoeugenol to vanillin (2013), Biosci. Biotechnol. Biochem., 77, 289-294 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.88 additional information not inhibitory or activating at 1 mM: Mg(II), Mn(II), Mo(II), NADH, FAD, or ascorbic acid, EDTA, 1,10-phenanthroline, and Tiron Pseudomonas nitroreducens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.88 expression in ERscherichia coli Pseudomonas nitroreducens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.88 H167A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens
1.13.11.88 H205A mutation of conserved His residue, 6.8% residual catalytic activity Pseudomonas nitroreducens
1.13.11.88 H218A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens
1.13.11.88 H282A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.88 Co2+ 1 mM, 40% of initial activity Pseudomonas nitroreducens
1.13.11.88 additional information not inhibitory or activating at 1 mM: Mg(II), Mn(II), Mo(II), NADH, FAD, or ascorbic acid, EDTA, 1,10-phenanthroline, and Tiron Pseudomonas nitroreducens
1.13.11.88 Ni2+ 1 mM, 49% of initial activity Pseudomonas nitroreducens
1.13.11.88 Zn2+ 1 mM, 35% of initial activity Pseudomonas nitroreducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.88 0.12
-
 isoeugenol pH 8.0, 30°C Pseudomonas nitroreducens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.88 Fe2+ presence of 0.7 mol iron per mol of enzyme Pseudomonas nitroreducens
1.13.11.88 Fe3+ 1 mM, 114% of initial activity Pseudomonas nitroreducens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.88 Pseudomonas nitroreducens C3VA26
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.88 4.2
-
 pH 8.0, 30°C Pseudomonas nitroreducens

Storage Stability

EC Number Storage Stability Organism
1.13.11.88 -70°C, 20mM Tris/HCl, 10% glycerol, and 1mM dithiothreitol, pH 8.0, stable for several months Pseudomonas nitroreducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.88 isoeugenol + O2
-
 Pseudomonas nitroreducens vanillin + acetaldehyde
-
 ?
1.13.11.88 additional information oxidative cleavage of isoeugenol by Iem is catalyzed via a monooxygenation reaction, and incorporation of the oxygen atom from O2 into vanillin is preferred over incorporation from water. Iem exhibits no activity toward any other of the phenylpropanoid and styrene compounds tested Pseudomonas nitroreducens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.88 ? x * 54000, SDS-PAGE Pseudomonas nitroreducens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.88 30
-
-
 Pseudomonas nitroreducens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.88 20
-
 stable for 30 min Pseudomonas nitroreducens
1.13.11.88 30
-
 30 min, 7% loss of activity Pseudomonas nitroreducens
1.13.11.88 50
-
 30 min, complete loss of activity Pseudomonas nitroreducens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.88 3.8
-
 isoeugenol pH 8.0, 30°C Pseudomonas nitroreducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.88 9
-
-
 Pseudomonas nitroreducens