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Literature summary extracted from

  • Dailey, H.A.; Gerdes, S.; Dailey, T.A.; Burch, J.S.; Phillips, J.D.
    Noncanonical coproporphyrin-dependent bacterial heme biosynthesis pathway that does not use protoporphyrin (2015), Proc. Natl. Acad. Sci. USA, 112, 2210-2215.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.98.1.1 0.72
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 0.0018
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis
4.99.1.9 0.0105
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis
-
 coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis
-
 protoporphyrin IX + H2O
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Bacillus subtilis
-
 Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Cutibacterium acnes
-
 Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Mycobacterium tuberculosis
-
 Fe-coproporphyrin III + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.15 Bacillus subtilis
-
-
-
1.3.98.5 Bacillota
-
-
-
4.98.1.1 Mycobacterium tuberculosis
-
-
-
4.99.1.9 Bacillus subtilis
-
-
-
4.99.1.9 Cutibacterium acnes
-
-
-
4.99.1.9 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.98.1.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2
-
 Bacillus subtilis coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2
-
 Bacillus subtilis protoporphyrin IX + H2O
-
 ?
4.98.1.1 protoporphyrin IX + Fe2+
-
 Mycobacterium tuberculosis protoheme IX + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Co2+ specific activity with Co2+ is 6fold higher than with Fe2+ Bacillus subtilis Co-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Co2+ specific activity with Co2+ is 2.8fold higher than with Fe2+ Mycobacterium tuberculosis Co-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Cu2+ specific activity with Cu2+ is 4.15old higher than with Fe2+ Bacillus subtilis Cu-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Cu2+ specific activity with Cu2+ is 5.35old higher than with Fe2+ Mycobacterium tuberculosis Cu-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Bacillus subtilis Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Cutibacterium acnes Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Mycobacterium tuberculosis Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Ni2+ specific activity with Ni2+ is 4fold lower than with Fe2+ Bacillus subtilis Ni-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Ni2+ specific activity with Ni2+ is 80% compared to activity with Fe2+ Mycobacterium tuberculosis Ni-coproporphyrin III + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.15 coproporphyrinogen oxidase
-
 Bacillus subtilis
1.3.3.15 hemY
-
 Bacillus subtilis
1.3.3.15 protoporphyrinogen oxidase
-
 Bacillus subtilis
4.98.1.1 hemH
-
 Mycobacterium tuberculosis
4.99.1.9 hemH
-
 Bacillus subtilis
4.99.1.9 hemH gene name. HemH and HemQ exist as a fusion protein Cutibacterium acnes
4.99.1.9 hemH
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.98.1.1 0.013
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 0.0078
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis
4.99.1.9 0.03
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.3.98.5 physiological function Actinobacteria and Firmicutes (high-GC and low-GC Gram-positive bacteria) are unable to synthesize protoporphyrin. Instead, they oxidize coproporphyrinogen to coproporphyrin, insert ferrous iron to make Fe-coproporphyrin (coproheme), and then decarboxylate coproheme to generate protoheme. This pathway is specified by three genes named hemY, hemH, and hemQ and is the most ancient heme synthesis pathway in the Eubacteria Bacillota

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.98.1.1 0.018
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 2.9
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 4.3
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis