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Literature summary extracted from

  • Bejugam, P.; Kuppili, R.; Singh, N.; Gadewal, N.; Chaganti, L.; Sastry, G.; Bose, K.
    Allosteric regulation of serine protease HtrA2 through novel non-canonical substrate binding pocket (2013), PLoS ONE, 8, e55416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.108
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 E292A residue involved in most of the peptide interactions, about 30% of wild-type catalytic efficiency Homo sapiens
3.4.21.108 E296A residue involved in most of the peptide interactions, about 90% of wild-type catalytic efficiency Homo sapiens
3.4.21.108 F16D residue involved in most of the peptide interactions, almost complete loss of catalytic activity Homo sapiens
3.4.21.108 G230A residue involved in most of the peptide interactions, about 12% of wild-type catalytic efficiency Homo sapiens
3.4.21.108 additional information mutant comprising N-terminal and serine protease domains, residues 1–210, displays about 20% of wild-type catalytic efficiency Homo sapiens
3.4.21.108 N216A/S219A residues involved in most of the peptide interactions, about 33% of wild-type catalytic efficiency Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.108 0.00302
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.00459
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.00468
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.00515
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.00543
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0093
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.00932
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens O43464
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 beta-casein + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.108 additional information allostery does regulate HtrA2 activity using a non-canonical selective binding pocket in HtrA2 which initiates signal propagation to the distal active site through a complex allosteric mechanism Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.108 0.000025
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0039
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0051
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0095
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0097
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0187
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.0204
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.108 0.0026
-
 beta-casein mutant F16D, pH 8.0, 37°C Homo sapiens
3.4.21.108 0.54
-
 beta-casein mutant G230A, pH 8.0, 37°C Homo sapiens
3.4.21.108 1.29
-
 beta-casein mutant comprising N-terminal and serine protease domains, pH 8.0, 37°C Homo sapiens
3.4.21.108 1.78
-
 beta-casein mutant N216A/S219A, pH 8.0, 37°C Homo sapiens
3.4.21.108 1.85
-
 beta-casein mutant E292A, pH 8.0, 37°C Homo sapiens
3.4.21.108 3.99
-
 beta-casein mutant E296A, pH 8.0, 37°C Homo sapiens
3.4.21.108 4.45
-
 beta-casein wild-type, pH 8.0, 37°C Homo sapiens