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Literature summary extracted from

  • Zurawa-Janicka, D.; Jarzab, M.; Polit, A.; Skorko-Glonek, J.; Lesner, A.; Gitlin, A.; Gieldon, A.; Ciarkowski, J.; Glaza, P.; Lubomska, A.; Lipinska, B.
    Temperature-induced changes of HtrA2(Omi) protease activity and structure (2013), Cell Stress Chaperones, 18, 35-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 F303W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
3.4.21.108 F331W mutation in proease domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
3.4.21.108 F331Y inactive Homo sapiens
3.4.21.108 I329N inactive Homo sapiens
3.4.21.108 L367W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
3.4.21.108 L377W mutation in PDZ domain, mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
3.4.21.108 R432L mutation in PDZ domain, significant increase in activity Homo sapiens
3.4.21.108 V226K mutation in protease domain, significant increase in activity Homo sapiens
3.4.21.108 V226K E429L salt link formation is no longer possible, the increase of activity observed for mutant V226K ceased to exist Homo sapiens
3.4.21.108 V226W mutation in proease domain, proteolytic activity similar to wild-type Homo sapiens
3.4.21.108 V325D inactive Homo sapiens
3.4.21.108 V364W mutation does not cause significant destabilizing or stabilizing effects on the thermal denaturation Homo sapiens
3.4.21.108 Y361W mutation in proease domain, proteolytic activity similar to wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.108 0.00158
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens O43464
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 HtrA2(Omi)
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.108 45
-
 activity increases about 14fold between 25 and 45 °C Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.108 20
-
 activity is practically negligible at 20 °C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.108 30
-
 upon an increase in temperature the HtrA2 structure relaxes, the PDZ-protease interface becomes more exposed to the solvent, and significant conformational changes involving both domains occur at and above 30 °C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.108 0.05
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.108 31.13
-
 (7-methoxycoumarin-4-yl)AIRRVSYSF-(5-amino-2-nitro)benzamide pH 8.0, 45°C Homo sapiens