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Literature summary extracted from

  • Kuan, Y.; Kao, C.; Chen, C.; Chen, C.; Hu, H.; Hsu, W.
    Biochemical characterization of a novel lysine racemase from Proteus mirabilis BCRC10725 (2011), Process Biochem., 46, 1914-1920.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.5 gene lyr, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Proteus mirabilis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.1.5 S394C site-directed mutagenesis, the mutant shows 1.5fold increased activity with L-arginine compared to the wild-type enzyme Proteus mirabilis
5.1.1.5 S394N site-directed mutagenesis, the mutant shows about 2.2fold increased activity with L-arginine compared to the wild-type enzyme Proteus mirabilis
5.1.1.5 S394T site-directed mutagenesis, the mutant shows 1.8fold increased activity with L-arginine compared to the wild-type enzyme Proteus mirabilis
5.1.1.5 S394Y site-directed mutagenesis, the mutant shows 2.1fold increased activity with L-arginine compared to the wild-type enzyme Proteus mirabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.5 hydroxylamine completely inhibition of the enzyme's L-Lys racemization at 5 mM, partially restored by the addition of pyridoxal 5'-phosphate Proteus mirabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.5 1 7 L-arginine pH 8.0, 50°C, recombinant mutant S394T Proteus mirabilis
5.1.1.5 1 2.5 L-arginine pH 8.0, 50°C, recombinant mutant S394C Proteus mirabilis
5.1.1.5 2 6 L-lysine pH 8.0, 50°C, recombinant mutant S394C Proteus mirabilis
5.1.1.5 2 6 L-lysine pH 8.0, 50°C, recombinant mutant S394N Proteus mirabilis
5.1.1.5 14.9
-
L-arginine pH 8.0, 50°C, recombinant wild-type enzyme Proteus mirabilis
5.1.1.5 17.1
-
L-arginine pH 8.0, 50°C, recombinant mutant S394N Proteus mirabilis
5.1.1.5 17.8
-
L-arginine pH 8.0, 50°C, recombinant mutant S394Y Proteus mirabilis
5.1.1.5 21.8
-
L-lysine pH 8.0, 50°C, recombinant wild-type enzyme Proteus mirabilis
5.1.1.5 25.2
-
L-lysine pH 8.0, 50°C, recombinant mutant S394T Proteus mirabilis
5.1.1.5 26.5
-
L-lysine pH 8.0, 50°C, recombinant mutant S394Y Proteus mirabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.5 L-lysine Proteus mirabilis
-
D-lysine
-
r
5.1.1.5 L-lysine Proteus mirabilis BCRC10725
-
D-lysine
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.5 Proteus mirabilis M4GGR9 gene lyr
-
5.1.1.5 Proteus mirabilis BCRC10725 M4GGR9 gene lyr
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.5 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Proteus mirabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.5 568
-
purified recombinant His-tagged enzyme, substrate L-arginine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 887
-
purified recombinant His-tagged mutant S394Y, substrate L-lysine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 980
-
purified recombinant His-tagged mutant S394C, substrate L-arginine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 1043
-
purified recombinant His-tagged mutant S394T, substrate L-arginine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 1185
-
purified recombinant His-tagged mutant S394Y, substrate L-arginine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 1200
-
purified recombinant His-tagged mutant S394N, substrate L-arginine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 1633
-
purified recombinant His-tagged mutant S394N, substrate L-lysine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 2356
-
purified recombinant His-tagged mutant S394C, substrate L-lysine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 2828
-
purified recombinant His-tagged enzyme, substrate L-lysine, pH 8.0, 50°C Proteus mirabilis
5.1.1.5 3127
-
purified recombinant His-tagged mutant S394T, substrate L-lysine, pH 8.0, 50°C Proteus mirabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.5 L-arginine
-
Proteus mirabilis D-arginine
-
r
5.1.1.5 L-arginine
-
Proteus mirabilis BCRC10725 D-arginine
-
r
5.1.1.5 L-lysine
-
Proteus mirabilis D-lysine
-
r
5.1.1.5 L-lysine
-
Proteus mirabilis BCRC10725 D-lysine
-
r
5.1.1.5 additional information enzyme residue S394 residue plays a crucial role in determining the preference of Lyr to lysine and arginine. No activity by wild-type and mutant enzyme with alanine, methionine, histidine, tryptophan, leucine, glutamine, asparagine and serine Proteus mirabilis ?
-
?
5.1.1.5 additional information enzyme residue S394 residue plays a crucial role in determining the preference of Lyr to lysine and arginine. No activity by wild-type and mutant enzyme with alanine, methionine, histidine, tryptophan, leucine, glutamine, asparagine and serine Proteus mirabilis BCRC10725 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.5 lyr
-
Proteus mirabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.5 50
-
recombinant His-tagged enzyme, substrate L-lysine Proteus mirabilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.5 10.83
-
L-arginine pH 8.0, 50°C, recombinant wild-type enzyme Proteus mirabilis
5.1.1.5 14.93
-
L-lysine pH 8.0, 50°C, recombinant mutant S394Y Proteus mirabilis
5.1.1.5 16.2
-
L-arginine pH 8.0, 50°C, recombinant mutant S394C Proteus mirabilis
5.1.1.5 17.45
-
L-arginine pH 8.0, 50°C, recombinant mutant S394T Proteus mirabilis
5.1.1.5 18.75
-
L-arginine pH 8.0, 50°C, recombinant mutant S394N Proteus mirabilis
5.1.1.5 19.67
-
L-arginine pH 8.0, 50°C, recombinant mutant S394Y Proteus mirabilis
5.1.1.5 31.25
-
L-lysine pH 8.0, 50°C, recombinant mutant S394N Proteus mirabilis
5.1.1.5 50.68
-
L-lysine pH 8.0, 50°C, recombinant mutant S394C Proteus mirabilis
5.1.1.5 55.43
-
L-lysine pH 8.0, 50°C, recombinant wild-type enzyme Proteus mirabilis
5.1.1.5 62.5
-
L-lysine pH 8.0, 50°C, recombinant mutant S394T Proteus mirabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.5 8 9 recombinant His-tagged enzyme, substrate L-lysine Proteus mirabilis

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.5 pyridoxal 5'-phosphate dependent on Proteus mirabilis