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Literature summary extracted from

  • Barney, B.M.; Wahlen, B.D.; Garner, E.; Wei, J.; Seefeldt, L.C.
    Differences in substrate specificities of five bacterial wax ester synthases (2012), Appl. Environ. Microbiol., 78, 5734-5745.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Marinobacter nauticus
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Acinetobacter baylyi
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Rhodococcus jostii
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Psychrobacter cryohalolentis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.75 expression in Escherichia coli Acinetobacter baylyi
2.3.1.75 expression in Escherichia coli Marinobacter nauticus
2.3.1.75 expression in Escherichia coli Psychrobacter cryohalolentis
2.3.1.75 expression in Escherichia coli Rhodococcus jostii
2.3.1.332 expressed in Escherichia coli JM109 cells Acinetobacter baylyi
2.3.1.332 expressed in Escherichia coli JM109 cells Marinobacter nauticus
2.3.1.332 expressed in Escherichia coli JM109 cells Psychrobacter cryohalolentis
2.3.1.332 expressed in Escherichia coli JM109 cells Rhodococcus jostii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.332 acyl-CoA + a medium-chain alcohol Psychrobacter cryohalolentis
-
 CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol Marinobacter nauticus
-
 CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol Acinetobacter baylyi
-
 CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol Rhodococcus jostii
-
 CoA + a medium-chain alcohol wax ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.75 Acinetobacter baylyi
-
-
-
2.3.1.75 Marinobacter nauticus
-
-
-
2.3.1.75 Psychrobacter cryohalolentis
-
-
-
2.3.1.75 Psychrobacter cryohalolentis K5
-
-
-
2.3.1.75 Rhodococcus jostii
-
-
-
2.3.1.332 Acinetobacter baylyi
-
-
-
2.3.1.332 Marinobacter nauticus
-
-
-
2.3.1.332 Psychrobacter cryohalolentis
-
-
-
2.3.1.332 Psychrobacter cryohalolentis K5
-
-
-
2.3.1.332 Rhodococcus jostii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.75 0.08
-
 substrate hexadecanol, pH 7.0, 22°C Acinetobacter baylyi
2.3.1.75 0.08
-
 substrate hexadecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
2.3.1.75 0.12
-
 substrate hexadecanol, pH 7.0, 22°C Rhodococcus jostii
2.3.1.75 0.24
-
 substrate hexadecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 0.68
-
 substrate hexadecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 0.83
-
 substrate dodecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
2.3.1.75 0.84
-
 substrate dodecanol, pH 7.0, 22°C Acinetobacter baylyi
2.3.1.75 3.37
-
 substrate dodecanol, pH 7.0, 22°C Rhodococcus jostii
2.3.1.75 4.96
-
 substrate dodecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 37.3
-
 substrate dodecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.75 decanol + palmitoyl-CoA and undecanol, best substrates Rhodococcus jostii decanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA and undecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA best substrate Acinetobacter baylyi dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Rhodococcus jostii dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 dodecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Marinobacter nauticus hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Marinobacter nauticus ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Acinetobacter baylyi ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis K5 ?
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA
-
 Acinetobacter baylyi tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis K5 tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA and dodecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Acinetobacter baylyi undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA and decanol, best substrates Rhodococcus jostii undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis undecanyl palmitate + CoASH
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 undecanyl palmitate + CoASH
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol
-
 Psychrobacter cryohalolentis CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol
-
 Marinobacter nauticus CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol
-
 Acinetobacter baylyi CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 acyl-CoA + a medium-chain alcohol
-
 Rhodococcus jostii CoA + a medium-chain alcohol wax ester
-
 ?
2.3.1.332 decanoyl-CoA + decanol about 90% activity compared to palmitoyl-CoA Psychrobacter cryohalolentis CoA + decyl decanoate
-
 ?
2.3.1.332 decanoyl-CoA + decanol about 40-45% activity compared to palmitoyl-CoA Marinobacter nauticus CoA + decyl decanoate
-
 ?
2.3.1.332 decanoyl-CoA + decanol about 10% activity compared to palmitoyl-CoA Acinetobacter baylyi CoA + decyl decanoate
-
 ?
2.3.1.332 decanoyl-CoA + decanol about 40% activity compared to palmitoyl-CoA Rhodococcus jostii CoA + decyl decanoate
-
 ?
2.3.1.332 lauroyl-CoA + decanol about 95% activity compared to palmitoyl-CoA Psychrobacter cryohalolentis CoA + decyl dodecanoate
-
 ?
2.3.1.332 lauroyl-CoA + decanol about 40-80% activity compared to palmitoyl-CoA Marinobacter nauticus CoA + decyl dodecanoate
-
 ?
2.3.1.332 lauroyl-CoA + decanol less than 5% activity compared to palmitoyl-CoA Acinetobacter baylyi CoA + decyl dodecanoate
-
 ?
2.3.1.332 lauroyl-CoA + decanol about 25% activity compared to palmitoyl-CoA Rhodococcus jostii CoA + decyl dodecanoate
-
 ?
2.3.1.332 myristoyl-CoA + decanol about 175% activity compared to palmitoyl-CoA Psychrobacter cryohalolentis CoA + decyl tetradecanoate
-
 ?
2.3.1.332 myristoyl-CoA + decanol about 110-140% activity compared to palmitoyl-CoA Marinobacter nauticus CoA + decyl tetradecanoate
-
 ?
2.3.1.332 myristoyl-CoA + decanol about 100% activity compared to palmitoyl-CoA Acinetobacter baylyi CoA + decyl tetradecanoate
-
 ?
2.3.1.332 myristoyl-CoA + decanol about 120% activity compared to palmitoyl-CoA Rhodococcus jostii CoA + decyl tetradecanoate
-
 ?
2.3.1.332 octanoyl-CoA + decanol about 30% activity compared to palmitoyl-CoA Psychrobacter cryohalolentis CoA + decyl octanoate
-
 ?
2.3.1.332 octanoyl-CoA + decanol about 10-20% activity compared to palmitoyl-CoA Marinobacter nauticus CoA + decyl octanoate
-
 ?
2.3.1.332 octanoyl-CoA + decanol less than 5% activity compared to palmitoyl-CoA Acinetobacter baylyi CoA + decyl octanoate
-
 ?
2.3.1.332 octanoyl-CoA + decanol less than 1% activity compared to palmitoyl-CoA Rhodococcus jostii CoA + decyl octanoate
-
 ?
2.3.1.332 palmitoyl-CoA + decanol 100% activity Psychrobacter cryohalolentis CoA + decyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + decanol 100% activity Marinobacter nauticus CoA + decyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + decanol 100% activity Acinetobacter baylyi CoA + decyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + decanol 100% activity Rhodococcus jostii CoA + decyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + dodecanol high activity Psychrobacter cryohalolentis CoA + dodecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + dodecanol high activity Marinobacter nauticus CoA + dodecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + dodecanol high activity Acinetobacter baylyi CoA + dodecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + dodecanol high activity Rhodococcus jostii CoA + dodecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + heptadecanol low activity Psychrobacter cryohalolentis CoA + hexadecyl heptadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + heptadecanol low activity Marinobacter nauticus CoA + hexadecyl heptadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + heptadecanol low activity Acinetobacter baylyi CoA + hexadecyl heptadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + heptadecanol low activity Rhodococcus jostii CoA + hexadecyl heptadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + hexadecanol low activity Psychrobacter cryohalolentis CoA + hexadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + hexadecanol low activity Marinobacter nauticus CoA + hexadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + hexadecanol low activity Acinetobacter baylyi CoA + hexadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + hexadecanol low activity Rhodococcus jostii CoA + hexadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + nonanol low activity Psychrobacter cryohalolentis CoA + nonyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + nonanol low activity Marinobacter nauticus CoA + nonyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + nonanol low activity Acinetobacter baylyi CoA + nonyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + nonanol low activity Rhodococcus jostii CoA + nonyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octadecanol low activity Psychrobacter cryohalolentis CoA + octadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octadecanol low activity Marinobacter nauticus CoA + octadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octadecanol low activity Acinetobacter baylyi CoA + octadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octadecanol low activity Rhodococcus jostii CoA + octadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octanol low activity Psychrobacter cryohalolentis CoA + octyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octanol low activity Marinobacter nauticus CoA + octyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octanol low activity Acinetobacter baylyi CoA + octyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + octanol low activity Rhodococcus jostii CoA + octyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + tetradecanol high activity Psychrobacter cryohalolentis CoA + tetradecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + tetradecanol high activity Marinobacter nauticus CoA + tetradecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + tetradecanol high activity Acinetobacter baylyi CoA + tetradecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + tetradecanol high activity Rhodococcus jostii CoA + tetradecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + undecanol high activity Psychrobacter cryohalolentis CoA + undecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + undecanol high activity Marinobacter nauticus CoA + undecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + undecanol high activity Acinetobacter baylyi CoA + undecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + undecanol high activity Rhodococcus jostii CoA + undecyl hexadecanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.75 Rha1
-
 Rhodococcus jostii
2.3.1.332 wax ester synthase/acyl-coenzyme A (acyl-CoA):diacylglycerol acyltransferase
-
 Psychrobacter cryohalolentis
2.3.1.332 wax ester synthase/acyl-coenzyme A (acyl-CoA):diacylglycerol acyltransferase
-
 Marinobacter nauticus
2.3.1.332 wax ester synthase/acyl-coenzyme A (acyl-CoA):diacylglycerol acyltransferase
-
 Acinetobacter baylyi
2.3.1.332 wax ester synthase/acyl-coenzyme A (acyl-CoA):diacylglycerol acyltransferase
-
 Rhodococcus jostii
2.3.1.332 WS/DGAT
-
 Psychrobacter cryohalolentis
2.3.1.332 WS/DGAT
-
 Marinobacter nauticus
2.3.1.332 WS/DGAT
-
 Acinetobacter baylyi
2.3.1.332 WS/DGAT
-
 Rhodococcus jostii