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Literature summary extracted from

  • Wu, Z.; Yang, Y.; Shaw, N.; Bhattacharya, S.; Yan, L.; West, K.; Roth, K.; Noy, N.; Qin, J.; Crabb, J.W.
    Mapping the ligand binding pocket in the cellular retinaldehyde binding protein (2003), J. Biol. Chem., 278, 12390-12396.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.315 expression in Hi-5 insect cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.315 0.0025
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11-cis-retinol-[cellular retinaldehyde binding protein] wild-type cellular retinaldehyde binding protein, recombinant wild-type RDH5, pH 7.5, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.315 Homo sapiens Q92781
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.315 11-cis-retinol-[cellular retinaldehyde binding protein] + NAD+ cellular retinaldehyde binding protein CRALBP serves as an 11-cis-retinol acceptor for the enzymatic isomerization of all-trans- to 11-cis-retinol and as a substrate carrier for 11-cis-retinol dehydrogenase RDH5. Altered kinetic parameters are observed for RDH5 oxidation of 11-cis-retinol bound to rCRALBP mutants M222A, M225A, and W244F, supporting impaired substrate carrier function. Data implicate Trp165, Met208, Met222, Met225, and Trp244 as components of the CRALBP ligand binding cavity Homo sapiens 11-cis-retinal-[cellular retinaldehyde binding protein] + NADH + H+
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