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Literature summary extracted from

  • Xia, L.; Ruppert, M.; Wang, M.; Panjikar, S.; Lin, H.; Rajendran, C.; Barleben, L.; Stoeckigt, J.
    Structures of alkaloid biosynthetic glucosidases decode substrate specificity (2012), ACS Chem. Biol., 7, 226-234.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.125 wild type and mutant enzyme E186Q in complex with the substrate 1,2-(S)-dihydroraucaffricine and secologanin, hanging drop vapor diffusion method, using 0.01-0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 4.5-5.0, 9-12% (w/v) PEG 4000 as precipitant buffer, and 20°C Rauvolfia serpentina

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.125 E186D inactive Rauvolfia serpentina
3.2.1.125 E186Q inactive Rauvolfia serpentina
3.2.1.125 E186Q/E420Q inactive Rauvolfia serpentina
3.2.1.125 E420Q the mutant shows 0.5% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 E476A the mutant shows 0.57% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 E476L the mutant shows 0.67% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 F485Y the mutant shows 24.24% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 H193A the mutant shows 43.66% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 S390G the mutant shows 30.91% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 T189A the mutant shows 63.94% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 W392A the mutant shows 1.21% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina
3.2.1.125 Y200A the mutant shows 4.6% activity with raucaffricine compared to the wild type enzyme Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 additional information enzyme activity is not inhibited by raucaffricine Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.12
-
 strictosidine pH and temperature not specified in the publication Rauvolfia serpentina
3.2.1.125 1.3
-
 Raucaffricine wild type enzyme, pH and temperature not specified in the publication Rauvolfia serpentina
3.2.1.125 1.8
-
 strictosidine wild type enzyme, pH and temperature not specified in the publication Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Rauvolfia serpentina Q8GU20
-
-
3.2.1.125 Rauvolfia serpentina
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 additional information the enzyme does not accept raucaffricine as substrate Rauvolfia serpentina ?
-
 ?
3.2.1.105 strictosidine + H2O
-
 Rauvolfia serpentina D-glucose + strictosidine aglycone
-
 ?
3.2.1.125 1,2-(S)-dihydro-raucaffricine + H2O 98% acivity compared to raucaffricine Rauvolfia serpentina 2beta(R)-1,2-dihydrovomilenine + D-glucose
-
 ?
3.2.1.125 additional information no activity with arbutin Rauvolfia serpentina ?
-
 ?
3.2.1.125 raucaffricine + H2O 100% activity Rauvolfia serpentina D-glucose + vomilenine
-
 ?
3.2.1.125 secologanin + H2O 0.24% acivity compared to raucaffricine Rauvolfia serpentina secologanin aglycone
-
 ?
3.2.1.125 strictosidine + H2O 1.18% acivity compared to raucaffricine Rauvolfia serpentina strictosidine aglycone + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.105 strictosidine beta-glucosidase
-
 Rauvolfia serpentina
3.2.1.105 strictosidine glucosidase
-
 Rauvolfia serpentina
3.2.1.125 raucaffricine glucosidase
-
 Rauvolfia serpentina
3.2.1.125 raucaffricine O-beta-D-glucosidase
-
 Rauvolfia serpentina