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Literature summary extracted from

  • Hartkamp, J.; Carpenter, B.; Roberts, S.G.
    The Wilms tumor suppressor protein WT1 is processed by the serine protease HtrA2/Omi (2010), Mol. Cell, 37, 159-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 recombinantly expressed Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 S306A mutant lacks proteolytic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.108 ucf-101 proteolysis of Wilms'tumor suppressor protein WT1 is prevented Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.108 additional information Homo sapiens HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 ?
-
 ?
3.4.21.108 Wilms' tumor suppressor 1 + H2O Homo sapiens HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 ?
-
 ?
3.4.21.108 Wilms' tumor suppressor protein WT1 + H2O Homo sapiens using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs ?
-
 ?
3.4.21.108 Wilms' tumor suppressor protein WT1 + H2O Mus musculus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens
-
-
-
3.4.21.108 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 embryonic fibroblast cell line
-
 Mus musculus
-
3.4.21.108 K-562 cell
-
 Homo sapiens
-
3.4.21.108 NCI-H1299 cell
-
 Homo sapiens
-
3.4.21.108 U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 additional information HtrA2 fails to cleave the Wilms' tumor suppressor 1 cofactors Par-4 and BASP1 Homo sapiens ?
-
 ?
3.4.21.108 Wilms' tumor suppressor 1 + H2O HtrA2 cleaves at, at least, two specific sites. Wilms' tumor suppressor 1 degradation by HtrA2 reveals two major proteolytic products of 20000 Da and 15000 Da, but the same samples immunoblotted with N-Ter antibody reveals major fragment sizes of 35000 Da and 30000 Da. The potential cleavage site that produces the 20000 Da fragment is at Val286, and the potential cleavage site producing the 15000 Da fragment is at Leu320 Homo sapiens ?
-
 ?
3.4.21.108 Wilms' tumor suppressor protein WT1 + H2O using a yeat two-hybrid assay WT1 is identified as a binding partner of HtrA2/Omi. HtrA2/Omi cleaves WT1 at multiple binding sites following the treatment of cells with cytotoxic drugs Homo sapiens ?
-
 ?
3.4.21.108 Wilms' tumor suppressor protein WT1 + H2O
-
 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 high temperature requirement protein A2
-
 Homo sapiens
3.4.21.108 HtrA2/Omi
-
 Homo sapiens
3.4.21.108 HtrA2/Omi
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.108 37
-
 assay at Homo sapiens
3.4.21.108 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.108 7.5
-
 assay at Homo sapiens
3.4.21.108 7.5
-
 assay at Mus musculus

Expression

EC Number Organism Comment Expression
3.4.21.108 Homo sapiens ablation of HtrA2 activity using RNAi in the human osteosarcoma U2OS cells prevents the cleavage of Wilms'tumor suppressor protein WT1 under apoptotic conditions down

General Information

EC Number General Information Comment Organism
3.4.21.108 malfunction in mouse embryonic HtrA2/omi knockout fibroblast cells the apoptosis-dependent cleavage of Wilms'tumor suppressor protein WT1 is prevented and expression of WT1 is upregulated Mus musculus
3.4.21.108 physiological function HtrA2/Omi cleave Wilms'tumor suppressor protein WT1 which causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis Homo sapiens
3.4.21.108 physiological function proteolysis of Wilms' tumor suppressor 1 by HtrA2 causes the removal of WT1 from its binding sites at gene promoters, leading to alterations in gene regulation that enhance apoptosis Homo sapiens