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Literature summary extracted from

  • Farjo, K.M.; Moiseyev, G.; Takahashi, Y.; Crouch, R.K.; Ma, J.X.
    RDH10 has 11-cis-retinol dehydrogenase activity and interacts with visual cycle proteins. (2009), Invest. Ophthalmol. Vis. Sci., 50, 5089-5097.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.315 cellular retinaldehyde-binding protein RDH10 oxidizes 11-cis-retinol to generate 11-cis-retinaldehyde in vitro in the presence of cellular retinaldehyde-binding protein Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.315 expressed in COS1 cells Homo sapiens
1.1.1.315 expression in Cos-1 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.315 0.00069
-
NADP+ pH 7.4, 37┬░C Homo sapiens
1.1.1.315 0.002
-
NAD+ pH 7.4, 37┬░C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.315 11-cis-retinol + NAD+ Homo sapiens RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity. RDH10 may function in the RPE retinoid visual cycle as an 11-cis-retinol dehydrogenase, and thereby partially compensate for the loss of RDH5 function in human patients with fundus albipunctatus 11-cis-retinal + NADH + H+
-
?
1.1.1.315 11-cis-retinol + NADP+ Homo sapiens RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity. RDH10 may function in the RPE retinoid visual cycle as an 11-cis-retinol dehydrogenase, and thereby partially compensate for the loss of RDH5 function in human patients with fundus albipunctatus 11-cis-retinal + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.315 Bos taurus Q8HZT6
-
-
1.1.1.315 Homo sapiens Q8IZV5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.315 retinal pigment epithelial cell RDH10 colocalizes with retinal pigment protein RPE65 and with cellular retinaldehyde-binding protein CRALBP in vivo Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.1.1.315 additional information
-
specific activity is sevenfold higher in the presence of NAD+ cofactor, 69.44 picomoles/mg membrane protein/min, than in the presence of NADP+ cofactor, 9.66 picomoles/mg membrane protein/min, pH 7.4, 37┬░C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.315 11-cis-retinol + NAD+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity. RDH10 may function in the RPE retinoid visual cycle as an 11-cis-retinol dehydrogenase, and thereby partially compensate for the loss of RDH5 function in human patients with fundus albipunctatus Homo sapiens 11-cis-retinal + NADH + H+
-
?
1.1.1.315 11-cis-retinol + NAD+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity Homo sapiens 11-cis-retinal + NADH + H+
-
?
1.1.1.315 11-cis-retinol + NADP+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity. RDH10 may function in the RPE retinoid visual cycle as an 11-cis-retinol dehydrogenase, and thereby partially compensate for the loss of RDH5 function in human patients with fundus albipunctatus Homo sapiens 11-cis-retinal + NADPH + H+
-
?
1.1.1.315 11-cis-retinol + NADP+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity Homo sapiens 11-cis-retinal + NADPH + H+
-
?
1.1.1.315 11-cis-retinol-[cellular retinaldehyde binding protein] + NAD+
-
Homo sapiens 11-cis-retinal-[cellular retinaldehyde binding protein] + NADH + H+
-
?
1.1.1.315 11-cis-retinol-[cellular retinaldehyde binding protein] + NADP+
-
Homo sapiens 11-cis-retinal-[cellular retinaldehyde binding protein] + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.315 More RDH10 physically interacts with RPE65 and with cellular retinaldehyde-binding protein CRALBP Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.315 11-cis-RDH
-
Homo sapiens
1.1.1.315 11-cis-retinol dehydrogenase
-
Homo sapiens
1.1.1.315 RDH10
-
Homo sapiens
1.1.1.315 RDH10
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.315 NAD+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity Homo sapiens
1.1.1.315 NAD+ enzyme can use both NAD+ and NADP+ Homo sapiens
1.1.1.315 NADP+ RDH10 can utilize both NAD+ and NADP+ as cofactors for 11-cis-retinol dehydrogenase activity. NAD+ cofactor confers more robust activity Homo sapiens
1.1.1.315 NADP+ enzyme can use both NAD+ and NADP+ Homo sapiens

General Information

EC Number General Information Comment Organism
1.1.1.315 physiological function reconstitution of the visual cycle in HEK-293A cells by co-expressing RDH10, cellular retinaldehyde-binding protein CRALBP, RPE-specific 65-kDa protein RPE65 and lecithin retinol acyltransferase LRAT leads to generation of 11-cis-retinol from all-trans-retinal Homo sapiens