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Literature summary extracted from

  • Kuninaka, S.; Iida, S.I.; Hara, T.; Nomura, M.; Naoe, H.; Morisaki, T.; Nitta, M.; Arima, Y.; Mimori, T.; Yonehara, S.; Saya, H.
    Serine protease Omi/HtrA2 targets WARTS kinase to control cell proliferation (2007), Oncogene, 26, 2395-2406.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.108 additional information apoptotic stimuli induce the activation of Omi and the consequent digestion of WTS by a caspase-independent mechanism Mus musculus
3.4.21.108 additional information activation of Omi by UV radiation Homo sapiens
3.4.21.108 staurosporine activates protease activity of Omi leading to cell death. Induces cell death in wild-type MEFs but not in caspase-9-deficient MEFs Mus musculus
3.4.21.108 staurosporine
-
 Homo sapiens
3.4.21.108 WTS activator of Omi protease Mus musculus

Application

EC Number Application Comment Organism
3.4.21.108 additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death Mus musculus
3.4.21.108 additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death Homo sapiens
3.4.21.108 additional information a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death synthetic construct

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 expressed in Escherichia coli (BL21) transformed with pRSET containing cDNA for full-length wild-type Omi or mutant synthetic construct

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 S306A inactive, has no effect on the abundance of WTS synthetic construct
3.4.21.108 Y361A mature Omi with PDZ domain mutation, cannot interact with C-terminus of WTS, fails to cleave full-length WTS synthetic construct

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.108 N-tosyllysine chloromethyl ketone
-
 Homo sapiens
3.4.21.108 N-tosyllysine chloromethyl ketone blocks ability of wild-type Omi to proteolyse WTS synthetic construct
3.4.21.108 siRNA reduces endogenous Omi expression in HeLa cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 mitochondrion
-
 Homo sapiens 5739
-
3.4.21.108 nucleus
-
 Homo sapiens 5634
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens
-
-
-
3.4.21.108 Mus musculus
-
-
-
3.4.21.108 synthetic construct
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.108 immunopurified from HEK-293 T cells Homo sapiens
3.4.21.108 on Ni2+ agarose beads synthetic construct

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 HEK-293T cell
-
 Homo sapiens
-
3.4.21.108 HeLa cell full length Omi present in mitochondria and nucleus Homo sapiens
-
3.4.21.108 L-929 cell
-
 Mus musculus
-
3.4.21.108 MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 WTS + H2O is a non-apoptotic substrate of Omi Mus musculus ?
-
 ?
3.4.21.108 WTS + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.108 WTS + H2O
-
 synthetic construct ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 HtrA2
-
 Mus musculus
3.4.21.108 HtrA2
-
 Homo sapiens
3.4.21.108 HtrA2
-
 synthetic construct
3.4.21.108 Omi
-
 Mus musculus
3.4.21.108 Omi
-
 Homo sapiens
3.4.21.108 Omi
-
 synthetic construct
3.4.21.108 Omi protease
-
 Mus musculus
3.4.21.108 Omi protease
-
 Homo sapiens
3.4.21.108 Omi protease
-
 synthetic construct
3.4.21.108 Omi/HtrA2
-
 Mus musculus
3.4.21.108 Omi/HtrA2
-
 Homo sapiens
3.4.21.108 Omi/HtrA2
-
 synthetic construct
3.4.21.108 serine protease Omi/HtrA2
-
 Mus musculus
3.4.21.108 serine protease Omi/HtrA2
-
 Homo sapiens
3.4.21.108 serine protease Omi/HtrA2
-
 synthetic construct