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Literature summary extracted from

  • Plun-Favreau, H.; Klupsch, K.; Moisoi, N.; Gandhi, S.; Kjaer, S.; Frith, D.; Harvey, K.; Deas, E.; Harvey, R.J.; McDonald, N.; Wood, N.W.; Martins, L.M.; Downward, J.
    The mitochondrial protease HtrA2 is regulated by Parkinsons disease-associated kinase PINK1 (2007), Nat. Cell Biol., 9, 1243-1252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.108 additional information phosphorylation of HtrA2 increases its proteolytic activtiy. PINK1 modulates the levels of phosphorylated HtrA2. Activation of MEKK3 induces efficient phosphorylation of both full-length and processed HtrA2 on Ser142. In vitro, p38 is also able to phosphorylate around Ser142 Homo sapiens

Application

EC Number Application Comment Organism
3.4.21.108 medicine HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. HtrA2 interacts with PINK1 and that both are components of the same stress-sensing pathway Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 TAP-tagged HtrA2 stably expressed in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 A141S mutation in HtrA2 identified in patients with Parkinson's disease Homo sapiens
3.4.21.108 G399S mutation in HtrA2 identified in patients with Parkinson's disease, ability of p38gamma to phosphorylate the peptide is decreased Homo sapiens
3.4.21.108 additional information HtrA2 knockout MEFs, no endogenous PINK1. Show decreased mitochondrial membrane potential Mus musculus
3.4.21.108 S142A considerably less phosphorylated by p38gamma in vitro. Markedly lower protease activity than its acidic counterpart Homo sapiens
3.4.21.108 S142D phospho-mimetic HtrA2-mutant, increases the basal ability of HtrA2 to cleave a fluorogenic substrate peptide by about 2fold Homo sapiens
3.4.21.108 S400A considerably less phosphorylated by p38gamma in vitro. Markedly lower protease activity than its acidic counterpart Homo sapiens
3.4.21.108 S400D phospho-mimetic HtrA2-mutant, increases the basal ability of HtrA2 to cleave a fluorogenic substrate peptide by about 3fold Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.108 additional information HtrA2 phosphorylation is decreased in brains of patients with Parkinson's disease carrying mutations in PINK1 (Y431H and C575R) Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 mitochondrion
-
 Homo sapiens 5739
-
3.4.21.108 mitochondrion
-
 Mus musculus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens
-
-
-
3.4.21.108 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.108 by gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 brain
-
 Homo sapiens
-
3.4.21.108 MEF cell
-
 Mus musculus
-
3.4.21.108 SH-SY5Y cell
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 Omi
-
 Homo sapiens
3.4.21.108 Omi
-
 Mus musculus
3.4.21.108 serine protease HtrA2
-
 Homo sapiens
3.4.21.108 serine protease HtrA2
-
 Mus musculus