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Literature summary extracted from

  • Yang, L.; Sun, M.; Sun, X.M.; Cheng, G.Z.; Nicosia, S.V.; Cheng, J.Q.
    Akt attenuation of the serine protease activity of HtrA2/Omi through phosphorylation of serine 212 (2007), J. Biol. Chem., 282, 10981-10987.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.108 additional information serine/threonine kinases Akt1 and Akt2 phosphorylate mitochondria-released HtrA2/Omi on serine 212 in vivo and in vitro, which results in attenuation of its serine protease activity and pro-apoptotic function. Akt phosphorylation of HtrA2/Omi at serine 212 is a critical event for attenuation of HtrA2/Omi cleaving X-linked inhibitor of apoptosis protein, although it does not affect HtrA2/Omi-X-linked inhibitor of apoptosis protein complex formation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 HtrA2/Omi cDNA cloned into pEGFP-C3 at KpnI/XbaI sites. HeLa cells transfected with GFP-fused premature or mature HtrA2/Omi together with or without wild-type and constitutively active Akt. HEK-293 cells transfected with pcDNA3-Myc-HtrA2/Omi together with or without wild-type and constitutively active Akt Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.108 S212A mutant with abolished HtrA2/Omi phosphorylation by Akt. It retains its serine protease activity and induces more apoptosis as compared with wild-type HtrA2/Omi Homo sapiens
3.4.21.108 S212D mutant mimicking phosphorylation. It has lost the protease activity and fails to induce programmed cell death Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.108 additional information Akt abrogates HtrA2/Omi pro-apoptotic function through phosphorylation of serine 212 in cytoplasm and inhibition of its release from the mitochondria in response to cisplatin treatment. Caspase inhibitor z-VAD-FMK reduces the mature HtrA2/Omi-induced cell death by ca. 45% Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 cytoplasm
-
 Homo sapiens 5737
-
3.4.21.108 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.108
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 OV2008 cell
-
 Homo sapiens
-
3.4.21.108 OVCAR-3 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 beta-casein + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.108 X-linked inhibitor of apoptosis protein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 HtrA2/Omi
-
 Homo sapiens
3.4.21.108 serine protease HtrA2/Omi
-
 Homo sapiens