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Literature summary extracted from

  • Eschbach, M.L.; Mueller, I.B.; Gilberger, T.W.; Walter, R.D.; Wrenger, C.
    The human malaria parasite Plasmodium falciparum expresses an atypical N-terminally extended pyrophosphokinase with specificity for thiamine (2006), Biol. Chem., 387, 1583-1591.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.6.2 medicine Malaria is a devastating and quite often deadly parasitic disease, resulting in significant public health problems in the tropics. The inevitable emergence of drug-resistant forms requires the continuous discovery and development of new antimalarials. These drugs should only target the parasite, with only harmless or no effects on the human host. Thus, ideal drug targets would be peculiarities in the parasite metabolism, such as vitamin B1 and B6 biosyntheses that are absent in its host. Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.2 0.062
-
 pyrithiamine
-
 Plasmodium falciparum
2.7.6.2 0.073
-
 thiamine thiamine monophosphate is not accepted Plasmodium falciparum
2.7.6.2 1.1
-
 ATP
-
 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.2 Mg2+ Optimal activity obtained by addition of 2-4 mM Mg2+. Plasmodium falciparum
2.7.6.2 Mn2+ Substitution of magnesium by Mn2+ decreases the enzyme activity by 42%. Plasmodium falciparum
2.7.6.2 Zn2+ Substitution of magnesium by Zn2+ decreases the enzyme activity by 85%. Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.2 38000
-
 for PfTPK3, SDS-PAGE analysis followed by Western blotting Plasmodium falciparum
2.7.6.2 43000
-
 for PfTPK2, SDS-PAGE analysis followed by Western blotting Plasmodium falciparum
2.7.6.2 47000
-
 for PfTPK, SDS-PAGE analysis followed by Western blotting Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.2 thiamine + ATP Plasmodium falciparum
-
 thiamine diphosphate + AMP
-
 ?
2.7.6.2 thiamine + ATP Saccharomyces cerevisiae
-
 thiamine diphosphate + AMP
-
 ?
2.7.6.2 thiamine + ATP Schizosaccharomyces pombe
-
 thiamine diphosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Plasmodium falciparum
-
-
-
2.7.6.2 Saccharomyces cerevisiae
-
-
-
2.7.6.2 Schizosaccharomyces pombe
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.2 0.009
-
 PfTPK3, with 4 mM magnesium ions in the enzyme assay Plasmodium falciparum
2.7.6.2 0.017
-
 PfTPK2, with 4 mM magnesium ions in the enzyme assay Plasmodium falciparum
2.7.6.2 0.017
-
 substrate: pyrithiamine, PfTPK Plasmodium falciparum
2.7.6.2 0.027
-
 PfTPK, with 4 mM magnesium ions in the enzyme assay Plasmodium falciparum
2.7.6.2 999
-
 no activity with thiochrome Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 pyrithiamine + ATP its specific activity is 30% lower in comparison to its natural substrate thiamine Plasmodium falciparum pyrithiamine diphosphate + AMP
-
 ?
2.7.6.2 thiamine + ATP
-
 Plasmodium falciparum thiamine diphosphate + AMP
-
 ?
2.7.6.2 thiamine + ATP
-
 Saccharomyces cerevisiae thiamine diphosphate + AMP
-
 ?
2.7.6.2 thiamine + ATP
-
 Schizosaccharomyces pombe thiamine diphosphate + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.2 dimer gel filtration using a calibrated Superdex S-200 column Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.7.6.2 Plasmodium falciparum thiamine pyrophosphokinase
-
 Plasmodium falciparum
2.7.6.2 pyrophosphokinase
-
 Plasmodium falciparum
2.7.6.2 pyrophosphokinase
-
 Saccharomyces cerevisiae
2.7.6.2 pyrophosphokinase
-
 Schizosaccharomyces pombe
2.7.6.2 thiamine pyrophosphokinase
-
 Plasmodium falciparum
2.7.6.2 thiamine pyrophosphokinase
-
 Saccharomyces cerevisiae
2.7.6.2 thiamine pyrophosphokinase
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.2 37
-
-
 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.2 8.5
-
 PfTPK Plasmodium falciparum