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Literature summary extracted from
- N-terminal truncation circumvents proteolytic degradation of the human HtrA2/Omi serine protease in Escherichia coli: rapid purification of a proteolytically active HtrA2/Omi (2004), Protein Expr. Purif., 33, 200-208.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.108 | barely expressed full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 | Homo sapiens |
| 3.4.21.108 | successful expression of full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 as compared to human HtrA2/Omi | Mus musculus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.108 | 28000 | 44000 | low molecular masses detected due to full-length HtrA2/Omi regions or amino acid residues that are very susceptible to a high degree of proteolytic events in Escherichia coli expression systems | Homo sapiens |
| 3.4.21.108 | 77000 | - |
- |
Mus musculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.108 | Homo sapiens | O43464 | - |
- |
| 3.4.21.108 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.108 | rapid purification of full-length HtrA2/Omi as a GST fusion under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads | Homo sapiens |
| 3.4.21.108 | rapid purification of GST-HtrA2/Omi fusion proteins under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.108 | brain | from fetus | Homo sapiens | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.108 | -70°C, Tris-HCl buffer, pH 7.6, 20% glycerol, several months | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.108 | beta-casein + H2O | - |
Homo sapiens | ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.108 | HtrA2/Omi | - |
Mus musculus |
| 3.4.21.108 | HtrA2/Omi serine protease | - |
Homo sapiens |
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