Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jain, J.C.; Groot Wassink, J.W.D.; Reed, D.W.; Underhill, E.W.
    Persistent co-purification of enzymes catalyzing the sequential glucosylation and sulfation steps in glucosinolate biosynthesis (1990), J. Plant Physiol., 136, 356-361.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.195 cytoplasm
-
Brassica juncea 5737
-
2.8.2.24 cytoplasm
-
Brassica juncea 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.195 44000
-
gel filtration Brassica juncea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica juncea
-
UDP + desulfoglucotropeolin
-
?
2.8.2.24 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate Brassica juncea
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 additional information Brassica juncea involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.195 Brassica juncea
-
cv. Cutlass
-
2.8.2.24 Brassica juncea
-
cv. Cutlass
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.195 co-purified with EC 2.8.2.24 Brassica juncea
2.8.2.24 persistently co-purified with EC 2.4.1.195 Brassica juncea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.24 cell culture derived from hypocotyls Brassica juncea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
Brassica juncea UDP + desulfoglucotropeolin
-
?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
2.8.2.24 additional information involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants Brassica juncea ?
-
?

Temperature Stability [┬░C]

EC Number Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
2.4.1.195 5 30 stable up to 30┬░C Brassica juncea
2.8.2.24 40
-
up to, stable Brassica juncea
2.8.2.24 45
-
t1/2: 1 h, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol Brassica juncea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.195 4 10 stable below pH 7.5 Brassica juncea
2.8.2.24 6
-
t1/2: 1 h, 4┬░C Brassica juncea
2.8.2.24 8
-
stable above Brassica juncea
2.8.2.24 10.5
-
t1/2: 1 h, 4┬░C Brassica juncea