Literature summary for 7.6.2.9 extracted from

Mahmood, N.A.; Biemans-Oldehinkel, E.; Patzlaff, J.S.; Schuurman-Wolters, G.K.; Poolman, B.
Ion specificity and ionic strength dependence of the osmoregulatory ABC transporter OpuA (2006), J. Biol. Chem., 281, 29830-29839.

Search for more literature for 7.6.2.9

Activating Compound

Activating Compound	Comment	Organism	Structure
dioleoyl-phosphatidylglycerol	needed for full osmoregulatory activity	Lactococcus lactis
Tetracaine	instantaneously activates inside-out oriented OpuA, reconstitued in membranes with 40% of anionic lipid, 1mM equally effective than 0.2 mM KCl	Lactococcus lactis

Application

Application	Comment	Organism
additional information	the cystathionine beta-synthase module in OpuA constitutes the ionic strength sensor whose activity is modulated by the C-terminal anionic tail	Lactococcus lactis

Protein Variants

Protein Variants	Comment	Organism
additional information	OpuAdelta12, anionic tail deletion mutant, Rb+ and Cs+ no longer inhibitory, more K+ needed for activation than for the wild-type	Lactococcus lactis

Inhibitors

Inhibitors	Comment	Organism	Structure
Cs+	potent inhibitor	Lactococcus lactis
Rb+	potent inhibitor	Lactococcus lactis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Lactococcus lactis	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	bivalent cations more effective activators than monovalent cations	Lactococcus lactis
K+	maximal enzyme activity reached with 250 mM KCl, no further effect in the presence of the protonophore SF6847	Lactococcus lactis
Li+	-	Lactococcus lactis
Mg2+	bivalent cations more effective activators than monovalent cations	Lactococcus lactis
Na+	-	Lactococcus lactis
NH4+	less effective than KCl, in the presence of the protonophore SF6847 much more effective	Lactococcus lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	Q9KIF7	strain Opu401	-
Lactococcus lactis IL1403	Q9KIF7	strain Opu401	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni2+-nitrilotriacetic acid resin	Lactococcus lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + glycine betaine/out	-	Lactococcus lactis	ADP + phosphate + glycine betaine/in	-	?
ATP + H2O + glycine betaine/out	-	Lactococcus lactis IL1403	ADP + phosphate + glycine betaine/in	-	?

Synonyms

Synonyms	Comment	Organism
OpuA	-	Lactococcus lactis

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Release 2023.1 (January 2023)