Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.9 extracted from

  • Laloknam, S.; Tanaka, K.; Buaboocha, T.; Waditee, R.; Incharoensakdi, A.; Hibino, T.; Tanaka, Y.; Takabe, T.
    Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity (2006), Appl. Environ. Microbiol., 72, 6018-6026.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.9

Activating Compound

Activating Compound Comment Organism Structure
additional information sucrose and sorbitol show no effect Aphanothece halophytica

Application

Application Comment Organism
additional information Na+-betaine symporter that contributes to the salt stress tolerance at alkaline pH Aphanothece halophytica

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pApBetT, transfered to Escherichia coli and then to MKH13 cells, overexpression in freshwater Synechococcus sp. strain PCC7942 cells Aphanothece halophytica

Inhibitors

Inhibitors Comment Organism Structure
Betaine aldehyde weakly inhibits betaine uptake Aphanothece halophytica
choline slightly inhibits betaine uptake Aphanothece halophytica
additional information betaine uptake not inhibited by gamma-aminobutyric acid, proline, glutamate, aspartate, glutamine, asparagine, glycine, sarcosine, dimethylglycine, lysine, histidine, alanine, leucine, isoleucine, serine, cysteine, threonine, valine, phenylalanine, tryptophan, and methionine Aphanothece halophytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00832
-
 betaine Aphanothece halophytica cells supplemented with 0.5 M NaCl Aphanothece halophytica
0.00941
-
 betaine Aphanothece halophytica cells supplemented with 2.0 M NaCl Aphanothece halophytica
0.0608
-
 betaine at pH 8.5, Aphanothece halophytica cells Aphanothece halophytica
0.0834
-
 betaine at pH 7.0, Aphanothece halophytica cells Aphanothece halophytica
0.0919
-
 betaine at pH 8.5, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica
0.128
-
 betaine at pH 7.0, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica
0.143
-
 betaine at pH 5.5, Aphanothece halophytica cells Aphanothece halophytica
0.272
-
 betaine at pH 5.5, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information K+, Li+, Cs+, Rb+, NH4+, Ca2+, and Mg2+ show no effect Aphanothece halophytica
Na+ enhances betaine uptake rates Aphanothece halophytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64660
-
 calculated from sequence analysis Aphanothece halophytica

Organism

Organism UniProt Comment Textmining
Aphanothece halophytica Q0PCR9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + betaine/out
-
 Aphanothece halophytica ADP + phosphate + betaine/in
-
 ?

Synonyms

Synonyms Comment Organism
BetTA.halophytica
-
 Aphanothece halophytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 for betaine uptake in Aphanothece halophytica cells Aphanothece halophytica

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Aphanothece halophytica