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Literature summary for 7.6.2.9 extracted from

  • Patzlaff, J.S.; van der Heide, T.; Poolman, B.
    The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuA (2003), J. Biol. Chem., 278, 29546-29551.
    View publication on PubMed
Search for more literature for 7.6.2.9

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibits ATPase activity Lactococcus lactis
additional information the enzyme is not inhibited by 400 mM glycine betaine on the trans side of the membrane. The enzyme is not inhibited by 250 mM carnitine Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 ATP 30ºC Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Lactococcus lactis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ significant reduction of glycine betaine efflux when 6 mM ADP/Mg2+ is added to the reaction Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis NZ9000
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] the mechanistica stoichiometry is 2 Lactococcus lactis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + glycine betaine/out no efflux of glycine betaine is observed in the absence of ATP Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
ATP + H2O + glycine betaine/out no efflux of glycine betaine is observed in the absence of ATP Lactococcus lactis NZ9000 ADP + phosphate + glycine betaine/in
-
 ?
additional information carnitine is no substrate for the enzyme Lactococcus lactis ?
-
 ?
additional information carnitine is no substrate for the enzyme Lactococcus lactis NZ9000 ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
12
-
 ADP 30ºC Lactococcus lactis