Activating Compound | Comment | Organism | Structure |
---|---|---|---|
anionic lipids | absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state | Lactococcus lactis | |
glycerol | at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample | Lactococcus lactis | |
additional information | sucrose and D-fructose have no effects on OpuA activity | Lactococcus lactis | |
phosphatidylethanolamine | non-bilayer lipid essential for high activity of OpuA | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K3PO4 | above 200 mM, inhibits ATPase activity | Lactococcus lactis | |
Tetracaine | low concentrations of the cationic amphipath decrease the activation of OpuA | Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Lactococcus lactis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K2SO4 | 100 mosmol/kg osmolality, stimulates OpuA activity | Lactococcus lactis | |
K3PO4 | 100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity | Lactococcus lactis | |
KCl | 100 mM, activates OpuA instantaneous and reversible | Lactococcus lactis | |
Na2SO4 | 100 mosmol/kg osmolality, stimulates OpuA activity | Lactococcus lactis | |
NaCl | 100 mosmol/kg osmolality, stimulates OpuA activity | Lactococcus lactis | |
Sodium phosphate | 100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography of solubilized membranes | Lactococcus lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | the osmotic activation of the transporter is set by the bulk charge in the lipid headgroup region of the membrane, indicating that electrostatic interactions between lipids and the transporter are intrinsic to the osmosensing mechanism | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg | Lactococcus lactis |
0.009 | - |
ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg | Lactococcus lactis |
0.018 | - |
ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg | Lactococcus lactis |
0.023 | - |
ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg | Lactococcus lactis |
0.095 | - |
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg | Lactococcus lactis |
0.099 | - |
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg | Lactococcus lactis |
0.117 | - |
ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg | Lactococcus lactis |
0.124 | - |
ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg | Lactococcus lactis |
0.146 | - |
ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg | Lactococcus lactis |
0.148 | - |
ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg | Lactococcus lactis |
0.18 | - |
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycine betaine/out | - |
Lactococcus lactis | ADP + phosphate + glycine betaine/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OpuA | - |
Lactococcus lactis |