Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.2 extracted from

  • Aurade, R.M.; Jayalakshmi, S.K.; Sreeramulu, K.
    P-glycoprotein ATPase from the resistant pest, Helicoverpa armigera: purification, characterization and effect of various insecticides on its transport function (2010), Biochim. Biophys. Acta, 1798, 1135-1143.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cypermethrin 0.1 mM, enhances ATPase activity by 40% Helicoverpa armigera
endosulfan 0.1 mM, stimulates ATPase activity by 30% Helicoverpa armigera
ethylparaoxon 0.1 mM, enhances ATPase activity by 20% Helicoverpa armigera
fenvalerate enhances ATPase activity Helicoverpa armigera
methylparathion 0.05 mM, stimulates ATPase activity by 30% and inhibits at higher concentrations Helicoverpa armigera
Monocrotophos 0.1 mM, stimulates ATPase activity by 25% Helicoverpa armigera

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Helicoverpa armigera
Ba2+ 1 mM, 90% loss of activity Helicoverpa armigera
methylparathion 0.05 mM, stimulates ATPase activity by 30% and inhibits at higher concentrations Helicoverpa armigera
additional information SDS, Triton X-100, urea, EGTA, EDTA, DTT and 1,10-phenanthroline have no significant effect on activity Helicoverpa armigera
N-ethylmaleimide 0.1 mM, 70% loss of activity Helicoverpa armigera
Pb2+ 3 mM, 77% loss of activity Helicoverpa armigera
probenecid 0.1 mM, 80% loss of activity Helicoverpa armigera
Sodium vanadate 0.01 mM, 90% loss of activity Helicoverpa armigera
vanadate 0.01 mM, the basal and fenvalerate or verapamil-stimulated ATPase activities are inhibited Helicoverpa armigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
ATP 22°C, pH 7.4, activation by ethylparaoxon (0.03 mM) or fenvalerate (0.03 mM) Helicoverpa armigera
1.2
-
ATP 22°C, pH 7.4, no activation Helicoverpa armigera

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 1 mM, increases the activity by 10%, respectively Helicoverpa armigera
NaCl 5 mM, increases the activity by 20%, respectively Helicoverpa armigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
x * 15000, SDS-PAGE Helicoverpa armigera

Organism

Organism UniProt Comment Textmining
Helicoverpa armigera
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Helicoverpa armigera

Purification (Commentary)

Purification (Comment) Organism
-
Helicoverpa armigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
22°C, pH 7.4 Helicoverpa armigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + tetramethylrosamine/in
-
Helicoverpa armigera ADP + phosphate + tetramethylrosamine/out
-
?
additional information the enzyme could cleave phosphoester bonds effectively from a broad range of substrates such as ATP, AMP, p-nitrophenylphosphate and beta-glycerophosphate Helicoverpa armigera ?
-
?

Subunits

Subunits Comment Organism
? x * 15000, SDS-PAGE Helicoverpa armigera

Synonyms

Synonyms Comment Organism
Ha-Pgp
-
Helicoverpa armigera
P-glycoprotein
-
Helicoverpa armigera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
Helicoverpa armigera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Helicoverpa armigera

General Information

General Information Comment Organism
physiological function the presence of Pgp could be one of the reasons for insecticide resistance in this pest Helicoverpa armigera