Protein Variants | Comment | Organism |
---|---|---|
C26A | no loss of ATPase activity | Escherichia coli |
C276A | no loss of ATPase activity | Escherichia coli |
C54T | mutated PotA protein loses both ATPase and spermidine uptake activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | inhibits ATPase activity of Pot A | Escherichia coli | |
PCMB | inhibits ATPase activity of Pot A | Escherichia coli | |
spermidine | inhibits ATPase activity of Pot A | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.385 | - |
ATP | ATPase activity of PotA | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | ATPase activity of PotA is dependent on Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PotA protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermidine/out + ATP + H2O | - |
Escherichia coli | spermidine/in + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is a ATP-binding cassette transporter | Escherichia coli |