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Literature summary for 7.6.2.1 extracted from

  • Roland, B.P.; Graham, T.R.
    Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase (2016), Proc. Natl. Acad. Sci. USA, 113, E4460-E4466 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.1

Protein Variants

Protein Variants Comment Organism
D256E the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
F213S the mutant shows increased activity with phosphatidylcholine, phosphatidylserine, and sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
I615M the mutant shows about wild type activity Saccharomyces cerevisiae
I615S the mutation significantly increases sphingomyelin and phosphatidylserine transport compared to the wild type enzyme Saccharomyces cerevisiae
I615T the mutant shows about wild type activity Saccharomyces cerevisiae
L242S the mutant shows reduced activity with phosphatidylcholine and wild type activity with sphingomyelin Saccharomyces cerevisiae
N220C the mutant shows wild type activity with phosphatidylcholine and increased activity with sphingomyelin Saccharomyces cerevisiae
N220S the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
N220S/L242S the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
N220T the mutant shows increased activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
N550S the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
T254A the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Saccharomyces cerevisiae 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + glycerophospholipid[side 1] Saccharomyces cerevisiae
-
 ADP + phosphate + glycerophospholipid[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + glycerophospholipid[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + glycerophospholipid[side 2]
-
 ?
ATP + H2O + phosphatidylcholine[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + phosphatidylcholine[side 2]
-
 ?
ATP + H2O + phosphatidylethanolamine[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + phosphatidylethanolamine[side 2]
-
 ?
ATP + H2O + phosphatidylserine[side 1] very low activity Saccharomyces cerevisiae ADP + phosphate + phosphatidylserine[side 2]
-
 ?
ATP + H2O + sphingomyelin[side 1] low activity Saccharomyces cerevisiae ADP + phosphate + sphingomyelin[side 2]
-
 ?
additional information no activity with phosphatidic acid Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Dnf1
-
 Saccharomyces cerevisiae
phospholipid flippase
-
 Saccharomyces cerevisiae
sphingomyelin flippase
-
 Saccharomyces cerevisiae