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Literature summary for 7.6.2.1 extracted from
- Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles (2006), Mol. Biol. Cell, 17, 1632-1642.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, removal of P4 ATPases Dnf1p and Dnf2p from budding yeast abolishes inward translocation of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled phosphatidylserine, phosphatidylethanolamine, and phosphatidylcholine across the plasma membrane and causes cell surface exposure of endogenous phosphatidylethanolamine, overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | Drs2p and Dnf3p are localized in the trans Golgi network | Saccharomyces cerevisiae | 5794 | - |
| plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
| secretory vesicle | post-Golgi, Drs2p is incorporated into the low-density class of secretory vesicles | Saccharomyces cerevisiae | 99503 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + phosphatidylethanolamine/in | Saccharomyces cerevisiae | selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylethanolamine/in | Saccharomyces cerevisiae EHY227 | selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | Saccharomyces cerevisiae | selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | Saccharomyces cerevisiae EHY227 | selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phospholipid/in | Saccharomyces cerevisiae | - |
ADP + phosphate + phospholipid/out | - |
? | |
| additional information | Saccharomyces cerevisiae | loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview | ? | - |
? | |
| additional information | Saccharomyces cerevisiae EHY227 | loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae EHY227 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + phosphatidylcholine/in | palmitoyl-(NBD-hexanoyl)-PC , selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylcholine/out | - |
? | |
| ATP + H2O + phosphatidylethanolamine/in | selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylethanolamine/in | palmitoyl-(NBD-hexanoyl)-PE , selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylethanolamine/in | selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae EHY227 | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylethanolamine/in | palmitoyl-(NBD-hexanoyl)-PE , selective ATP-dependent transport/flip of phosphatidylethanolamine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae EHY227 | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | palmitoyl-(NBD-hexanoyl)-PS , selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport/flip of phosphatidylserine from the outer to the inner monolayer of the cell membrane and post-Golgi secretory vesicles to maintain lipid asymmetry | Saccharomyces cerevisiae EHY227 | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phospholipid/in | - |
Saccharomyces cerevisiae | ADP + phosphate + phospholipid/out | - |
? | |
| additional information | loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | no transport of (NBD-hexanoyl)-sphingosine-1-phosphocholine | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles, overview | Saccharomyces cerevisiae EHY227 | ? | - |
? | |
| additional information | no transport of (NBD-hexanoyl)-sphingosine-1-phosphocholine | Saccharomyces cerevisiae EHY227 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminophospholipid translocase | - |
Saccharomyces cerevisiae |
| APLT | - |
Saccharomyces cerevisiae |
| Dnf3p | - |
Saccharomyces cerevisiae |
| Drs2p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
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