Literature summary for 7.6.2.1 extracted from

Tyurina, Y.Y.; Basova, L.V.; Konduru, N.V.; Tyurin, V.A.; Potapovich, A.I.; Cai, P.; Bayir, H.; Stoyanovsky, D.; Pitt, B.R.; Shvedova, A.A.; Fadeel, B.; Kagan, V.E.
Nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment: implications for the resolution of inflammation (2007), J. Biol. Chem., 282, 8498-8509.

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Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	APLT is sensitive to redox modifications of its sulfhydryl groups, macrophages can directly participate in apoptotic cell clearance by S-nitrosylation/oxidation and inhibition of APLT causing PS externalization, reversible by DTT, overview, no inhibition and phagocytosis stimulation by S-nitrosoglutathione	Homo sapiens
S-nitroso-L-cysteine-ethyl ester	i.e. SNCEE, causes intracellular and extracellular trans-nitrosylation of proteins, respectively. Nitrosative stress inhibits the enzyme, and SNCEE causes significant S-nitrosylation/oxidation of thiols in HL-60 cells. SNCEE also strongly inhibites APLT, activated scramblase, and causes PS externalization, reversible by DTT	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	cytosolic leaflet of the plasma membrane	Homo sapiens	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	-	Homo sapiens
Mg2+	-	Homo sapiens
additional information	addition of Ca2+ to the incubation medium does not cause a significant increase in the scramblase activity	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme SCRM-1 catalyzes the externalization of phosphatidylserine to the cell surface from the inner leaflet of the plasma membrane, nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment, involved in inflammation, reversible by DTT, overview, macrophages can directly participate in apoptotic cell clearance by S-nitrosylation/oxidation and inhibition of APLT causing PS externalization, reversible by DTT	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HL-60 cell	promyelocytic leukemia cells	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme SCRM-1 catalyzes the externalization of phosphatidylserine to the cell surface from the inner leaflet of the plasma membrane, nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment, involved in inflammation, reversible by DTT, overview, macrophages can directly participate in apoptotic cell clearance by S-nitrosylation/oxidation and inhibition of APLT causing PS externalization, reversible by DTT	Homo sapiens	?	-	?
additional information	the enzyme SCRM-1 catalyzes the externalization of phosphatidylserine to the cell surface from the inner leaflet of the plasma membrane	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
aminophospholipid translocase	-	Homo sapiens
APLT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)