Literature summary for 7.6.2.1 extracted from

Camberg, J.L.; Johnson, T.L.; Patrick, M.; Abendroth, J.; Hol, W.G.; Sandkvist, M.
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids (2007), EMBO J., 26, 19-27.

Search for more literature for 7.6.2.1

Activating Compound

Activating Compound	Comment	Organism	Structure
acidic phospholipids	acidic phospholipids, specifically cardiolipin, bind the co-purified EpsE/cyto-EpsL complex and stimulate its ATPase activity 30-130fold, whereas the activity of EpsE alone is unaffected. Removal of the last 11 residues, residues 243-253, from cyto-EpsL prevented cardiolipin binding as well as stimulation of the ATPase activity of EpsE	Vibrio cholerae serotype O1
cardiolipin	binds the co-purified EpsE/cyto-EpsL complex and stimulates its ATPase activity 30-130fold, whereas the activity of EpsE alone is unaffected. Removal of the last 11 residues, residues 243-253, from cyto-EpsL prevents cardiolipin binding as well as stimulation of the ATPase activity of EpsE	Vibrio cholerae serotype O1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	EpsE is a cytoplasmic component of the type II secretion system	Vibrio cholerae serotype O1	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Vibrio cholerae serotype O1

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + cholera toxin/in	through ATP hydrolysis and an interaction with the cytoplasmic membrane protein EpsL, EpsE supports secretion of cholera toxin across the outer membrane	Vibrio cholerae serotype O1	ADP + phosphate + cholera toxin/out	-	?

Synonyms

Synonyms	Comment	Organism
EpsE	-	Vibrio cholerae serotype O1

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Release 2023.1 (January 2023)