Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | rat liver contains two populations, DEPC-sensitive and DEPC-insensitive, of flippases, which can be in a different of DEPC sensitivity or be two different proteins | Rattus norvegicus | |
NEM | rat liver contains two populations of, NEM-sensitive and NEM-insensitive, flippases, which can be in a different of NEM sensitivity or be two different proteins | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Rattus norvegicus | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process | ? | - |
? | |
phospholipid/in | Rattus norvegicus | - |
phospholipid/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from liver endoplasmic reticulum | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution of the purified native enzyme in proteoliposomes using phospholipids and Triton X-100 at pH 7.5 and 22°C | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
aminophospholipid translocase assay using dithionite reduction by 4-nitrobenzo-2-oxa-1,3-diazole-labeled phospholipids, dithionate does not permeate membranes | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process | Rattus norvegicus | ? | - |
? | |
phosphatidylcholine/in | proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum | Rattus norvegicus | phosphatidylcholine/out | - |
? | |
phosphatidylethanolamine/in | proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum | Rattus norvegicus | phosphatidylethanolemine/out | - |
? | |
phosphatidylserine/in | proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum | Rattus norvegicus | phosphatidylserine/out | - |
? | |
phospholipid/in | - |
Rattus norvegicus | phospholipid/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerophospholipid flippase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |