Literature summary for 7.6.2.1 extracted from

Pomorski, T.; Lombardi, R.; Riezman, H.; Devaux, P.F.; van Meer, G.; Holthuis, J.C.M.
Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis (2003), Mol. Biol. Cell, 14, 1240-1254.

Cloned(Commentary)

Cloned (Comment)	Organism
Creation and study of strains lacking Dnf1, Dnf2 or both Dnf1 and Dnf2. The double mutant is defective in the inward translocalisation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane. The loss of both Dnf1 and Dnf2 leads to an increased cell surface exposure of endogenous aminophospholipids. The double mutant is sensitive to low concentrations of Co2+, Ni2+, Zn2+, and Mn2+	Saccharomyces cerevisiae

Cloned (Comment)

Organism

Creation and study of strains lacking Dnf1, Dnf2 or both Dnf1 and Dnf2. The double mutant is defective in the inward translocalisation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane. The loss of both Dnf1 and Dnf2 leads to an increased cell surface exposure of endogenous aminophospholipids. The double mutant is sensitive to low concentrations of Co2+, Ni2+, Zn2+, and Mn2+

Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	Dnf1p and Dnf2p	Saccharomyces cerevisiae	5886	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Saccharomyces cerevisiae	The enzyme is essential for inward translocation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane.	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	The enzyme is essential for inward translocation of NBD-labeled phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine across the plasmamembrane.	Saccharomyces cerevisiae	?	-	?