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Literature summary for 7.6.2.1 extracted from
- Identification and purification of aminophospholipid flippases (2000), Biochim. Biophys. Acta, 1486, 108-127.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | partially stimulates | Homo sapiens |  |
| F- | - |
Homo sapiens | |
| glycerophosphoserine | prevents transport of phosphatidylserine | Homo sapiens | |
| GPS | - |
Homo sapiens | |
| phenylacylbromide | - |
Homo sapiens | |
| Phenylglyoxal | - |
Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.0002 mM, inhibits from the cytofacial surface | Homo sapiens | |
| NEM | - |
Homo sapiens | |
| vanadate | - |
Bos taurus | |
| vanadate | inhibits the enzyme from the cytofacial surface | Homo sapiens | |
| vanadyl ion | inhibits the enzyme from the extracellular surface | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | plasma membrane | Homo sapiens | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Bos taurus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Homo sapiens | role in aminophospholipid transport | ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta | epithelial cells | Bos taurus | - |
| brain | - |
Bos taurus | - |
| brain | synaptosomes | Homo sapiens | - |
| epithelial cell | - |
Homo sapiens | - |
| erythrocyte | - |
Homo sapiens | - |
| fibroblast | - |
Homo sapiens | - |
| hepatocyte | - |
Homo sapiens | - |
| lymphocyte | - |
Homo sapiens | - |
| pheochromocytoma cell | - |
Homo sapiens | - |
| spermatozoon | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Bos taurus | ADP + phosphate | - |
? | |
| ATP + H2O | transport of ethanolamine at a rate 10fold slower than phosphatidylserine | Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | transport of phosphatidylserine | Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | the protein serves primarily to prevent the accumulation of phosphatidylserine on the outer surface of the cell | Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | transport of N-methylphosphatidylserine | Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | role in aminophospholipid transport | Homo sapiens | ADP + phosphate | - |
? | |
| dATP + H2O | - |
Homo sapiens | dADP + H2O | - |
? | |
| additional information | flippase recognizes multiple structural elements in its preferred lipid substrate. The lipid structural elements include a primary amine group, limited headgroup size and and sn-1,2-glycerol backbone. The presence of carboxyl group accelerates transport, but is not required for activity | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, SDS-PAGE | Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
below 4°C transport of phospholipids is inhibited in both erythrocytes and fibroblasts, warming to 7°C activates transport in fibroblasts | Homo sapiens |
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