Literature summary for 7.5.2.1 extracted from

Licht, A.; Bommer, M.; Werther, T.; Neumann, K.; Hobe, C.; Schneider, E.
Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF (2019), Res. Microbiol., 170, 1-12 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
maltose	3fold stimulation of ATPase activity at 0.6 mM	Bdellovibrio bacteriovorus
maltose-binding protein	-	Bdellovibrio bacteriovorus
maltotetraose	5fold stimulation of ATPase activity at 0.6 mM	Bdellovibrio bacteriovorus
maltotriose	4fold stimulation of ATPase activity at 0.6 mM	Bdellovibrio bacteriovorus
additional information	not stimulated by D-glucose, alpha-cyclodextrin, beta-cyclodextrin, and gamma-cyclodextrin	Bdellovibrio bacteriovorus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 T1 DE3 (pLysS) cells	Bdellovibrio bacteriovorus

Crystallization (Commentary)

Crystallization (Comment)	Organism
MalE domain, sitting drop vapor diffusion method, using 100 mM BisTris, pH 5.5, 0.2 M LiSO4, 25% (w/v) PEG 3350	Bdellovibrio bacteriovorus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bdellovibrio bacteriovorus	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + maltose-[maltose-binding protein][side 1]	Bdellovibrio bacteriovorus	-	ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]	-	?
ATP + H2O + maltose-[maltose-binding protein][side 1]	Bdellovibrio bacteriovorus HD100	-	ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]	-	?

Organism

Organism	UniProt	Comment	Textmining
Bdellovibrio bacteriovorus	Q6MNM0	-	-
Bdellovibrio bacteriovorus HD100	Q6MNM0	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon-HiTrap resin column chromatography, and Superdex 200 gel filtration	Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + maltose-[maltose-binding protein][side 1]	-	Bdellovibrio bacteriovorus	ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]	-	?
ATP + H2O + maltose-[maltose-binding protein][side 1]	-	Bdellovibrio bacteriovorus HD100	ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]	-	?

Subunits

Subunits	Comment	Organism
?	1 * 66000 + 1 * 25000 + 2 * 42000, SDS-PAGE	Bdellovibrio bacteriovorus

Synonyms

Synonyms	Comment	Organism
MalFGK2	-	Bdellovibrio bacteriovorus
maltose/maltodextrin ABC transporter	-	Bdellovibrio bacteriovorus

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Release 2023.1 (January 2023)