Literature summary for 7.4.2.8 extracted from

Rule, C.S.; Patrick, M.; Camberg, J.L.; Maricic, N.; Hol, W.G.; Sandkvist, M.
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE (2016), MicrobiologyOpen, 5, 870-882 .

Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Vibrio cholerae

Protein Variants

Protein Variants	Comment	Organism
C397S/C400S	inactive	Vibrio cholerae
C400S	the mutant is almost inactive	Vibrio cholerae
C430S	the mutant is almost inactive	Vibrio cholerae
C430S/C433S	the mutant is almost inactive	Vibrio cholerae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Vibrio cholerae	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	zinc is essential for activity and function of the enzyme	Vibrio cholerae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + cholera toxin[side 1]	Vibrio cholerae	-	ADP + phosphate + cholera toxin[side 2]	-	?
ATP + H2O + cholera toxin[side 1]	Vibrio cholerae ATCC 39315	-	ADP + phosphate + cholera toxin[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae	P37093	serotype TRH7000	-
Vibrio cholerae ATCC 39315	P37093	serotype TRH7000	-

Purification (Commentary)

Purification (Comment)	Organism
Talon cobalt resin column chromatography and Superose 6 gel filtration	Vibrio cholerae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + cholera toxin[side 1]	-	Vibrio cholerae	ADP + phosphate + cholera toxin[side 2]	-	?
ATP + H2O + cholera toxin[side 1]	-	Vibrio cholerae ATCC 39315	ADP + phosphate + cholera toxin[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE	Vibrio cholerae

Synonyms

Synonyms	Comment	Organism
Eps	-	Vibrio cholerae
T2S ATPase	-	Vibrio cholerae

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Release 2023.1 (January 2023)