Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Geobacillus thermodenitrificans |
Crystallization (Comment) | Organism |
---|---|
N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C, sitting drop vapor diffusion method, using 0.2 M magnesium formate and 20% (w/v) PEG3350 | Geobacillus thermodenitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Geobacillus thermodenitrificans | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + cellular protein[side 1] | Geobacillus thermodenitrificans | - |
ADP + phosphate + cellular protein[side 2] | - |
? | |
ATP + H2O + cellular protein[side 1] | Geobacillus thermodenitrificans NG80-2 | - |
ADP + phosphate + cellular protein[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4IKE7 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A4IKE7 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography and Superdex 75 gel filtration | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + cellular protein[side 1] | - |
Geobacillus thermodenitrificans | ADP + phosphate + cellular protein[side 2] | - |
? | |
ATP + H2O + cellular protein[side 1] | - |
Geobacillus thermodenitrificans NG80-2 | ADP + phosphate + cellular protein[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 169000, recombinant full-length enzyme, SDS-PAGE | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
EssC | the extreme C-terminal ATPase domain of protein EssC is essential for its activity as a key part of the type VII secretion system, whereas D2 and FHA domains are required for the production of a stable and functional protein | Geobacillus thermodenitrificans |