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Literature summary for 7.4.2.8 extracted from

  • Zoltner, M.; Ng, W.M.; Money, J.J.; Fyfe, P.K.; Kneuper, H.; Palmer, T.; Hunter, W.N.
    EssC domain structures inform on the elusive translocation channel in the type VII secretion system (2016), Biochem. J., 473, 1941-1952 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Geobacillus thermodenitrificans

Crystallization (Commentary)

Crystallization (Comment) Organism
N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C, sitting drop vapor diffusion method, using 0.2 M magnesium formate and 20% (w/v) PEG3350 Geobacillus thermodenitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Geobacillus thermodenitrificans 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + cellular protein[side 1] Geobacillus thermodenitrificans
-
ADP + phosphate + cellular protein[side 2]
-
?
ATP + H2O + cellular protein[side 1] Geobacillus thermodenitrificans NG80-2
-
ADP + phosphate + cellular protein[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4IKE7
-
-
Geobacillus thermodenitrificans NG80-2 A4IKE7
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography and Superdex 75 gel filtration Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + cellular protein[side 1]
-
Geobacillus thermodenitrificans ADP + phosphate + cellular protein[side 2]
-
?
ATP + H2O + cellular protein[side 1]
-
Geobacillus thermodenitrificans NG80-2 ADP + phosphate + cellular protein[side 2]
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 169000, recombinant full-length enzyme, SDS-PAGE Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
EssC the extreme C-terminal ATPase domain of protein EssC is essential for its activity as a key part of the type VII secretion system, whereas D2 and FHA domains are required for the production of a stable and functional protein Geobacillus thermodenitrificans