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Literature summary for 7.4.2.8 extracted from
- Kinetic characterization of the Shigella type three secretion system ATPase Spa47 using alpha-32P ATP (2018), Bio Protoc., 8, e3074 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Shigella flexneri |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | evaluation of a method for determination of enzyme kinetics of Spa47 using a direct alpha-32P ATPase assay, overview | Shigella flexneri |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Shigella flexneri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Shigella flexneri | - |
ADP + phosphate | - |
? | |
| ATP + H2O + cellular protein[side 1] | Shigella flexneri | - |
ADP + phosphate + cellular protein[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shigella flexneri | - |
- |
- |
| Shigella flexneri | Q6XVW8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Shigella flexneri | ADP + phosphate | - |
? | |
| ATP + H2O + cellular protein[side 1] | - |
Shigella flexneri | ADP + phosphate + cellular protein[side 2] | - |
? | |
| additional information | radioactive 32P ATP substrate is hydrolyzed by the enzyme, producing either ADP and 32P (inorganic phosphate) or alpha-32P ADP and inorganic phosphate from gamma-32P ATP and alpha-32P ATP, respectively. Determination of enzyme kinetics of purified Spa47 using a direct alpha-32P ATPase assay. Though this method does not readily support real-time monitoring of product formation, it carries many inherent advantages including high signal-to-noise, capability with essentially any buffer conditions required to support enzyme stability, and no downstream coupling reactions required for product detection/quantitation, overview | Shigella flexneri | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATPase | - |
Shigella flexneri |
| Spa47 | - |
Shigella flexneri |
| type three secretion system ATPase | - |
Shigella flexneri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Shigella flexneri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the Shigella protein Spa47 is an ATPase that supports protein secretion through its specialized type three secretion apparatus (T3SA), supporting infection of human host cells | Shigella flexneri |
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Release 2023.1 (January 2023)
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