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Literature summary for 7.4.2.8 extracted from
- Structural features reminiscent of ATP-driven protein translocases are essential for the function of a type III secretion-associated ATPase (2015), J. Bacteriol., 197, 3007-3014.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene invC, recombinant expression of His10-FLAG3-tagged InvC/FLAG3-tagged OrgB complexes in Escherichia coli strain BL21(DE3) | Salmonella enterica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E383A | site-directed mutagenesis, the mutant shows almost unaltered protein secretion activity compared to the wild-type | Salmonella enterica |
| E384A | site-directed mutagenesis, the mutant shows reduced protein secretion activity compared to the wild-type | Salmonella enterica |
| E384A/Y385A | site-directed mutagenesis, the mutant shows reduced protein secretion activity compared to the wild-type | Salmonella enterica |
| E384A/Y385A/G388A | site-directed mutagenesis, the mutant shows markedly reduced protein secretion activity and reduced ATPase activity compared to the wild-type | Salmonella enterica |
| G388A | site-directed mutagenesis, the mutant shows slightly reduced protein secretion activity compared to the wild-type | Salmonella enterica |
| K165E | site-directed mutagenesis, InvC ATPase inactive enzyme mutant | Salmonella enterica |
| Y385A | site-directed mutagenesis, the Salmonelly typhimurium mutant strain expressing InvCY385A shows a marked defect in its ability to secrete the effector proteins SptP and SopB, while expression of the substrate proteins is unaffected by introduction of mutations in InvC, the mutant shows reduced ATPase activity compared to the wild-type | Salmonella enterica |
| Y385W | site-directed mutagenesis, the mutant shows reduced ATPase activity compared to the wild-type | Salmonella enterica |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Salmonella enterica |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Salmonella enterica | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Salmonella enterica SL1344 | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | A0A0H3NGZ8 | serovar Typhimurium, gene invC | - |
| Salmonella enterica SL1344 | A0A0H3NGZ8 | serovar Typhimurium, gene invC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His10-FLAG3-tagged InvC/FLAG3-tagged OrgB complexes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Salmonella enterica | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Salmonella enterica SL1344 | ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | a two-helix-finger motif and a conserved loop located at the entrance of and within the predicted pore formed by the hexameric ATPase, structure modeling | Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| InvC | - |
Salmonella enterica |
| T3SS ATPase | - |
Salmonella enterica |
| type III secretion-associated ATPase | - |
Salmonella enterica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salmonella enterica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Salmonella enterica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme contains a two-helix-finger motif at the entrance of the modeled central pore of the InvC hexamer. The motif is highly conserved among type III secretion-associated ATPases, including those associated with the flagellar assembly apparatus. In addition to the tyrosine residue (Tyr385 in InvC) located at the center of the loop, other residues within the loop are highly conserved, such as Gly383, Glu384, and Gly388. Similarities between type III secretion ATPases and other ATP-driven protein translocases/unfoldases | Salmonella enterica |
| additional information | structure-function relationship, modeling based on the crystal structures of EscN, a T3SS ATPase from enteropathogenic Escherichia coli, PDB IDs 2OBM and 2OBL, and a crystal structure of the F1 ATPase hexamer, PDB ID 1BMF, overview. K165 is the catalytic residue of the ATPase | Salmonella enterica |
| physiological function | a two-helix-finger motif and a conserved loop located at the entrance of and within the predicted pore formed by the hexameric ATPase are essential for the ATP-driven protein translocase InvC function in the type III secretion system. Type III secretion machines are essential for the virulence or symbiotic relationships of many bacteria. An essential component of these machines is a highly conserved ATPase, which is necessary for the recognition and secretion of proteins destined to be delivered by the type III secretion pathway, e.g. secretion of the regulatory protein InvJ (early substrate), the translocases SipB, SipC, and SipD (middle substrate), and the effector proteins SptP and SopB (late substrates) | Salmonella enterica |
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