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Literature summary for 7.4.2.8 extracted from

  • Lorenz, C.; Buettner, D.
    Functional characterization of the type III secretion ATPase HrcN from the plant pathogen Xanthomonas campestris pv. vesicatoria (2009), J. Bacteriol., 191, 1414-1428.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
gene hrcN, cloned from strain 85-10, co-expression with c-Myc-tagged hrcL in Escherichia coli strain BL21(DE3), expression of Strep-tagged HrcN and Strep-tagged HrcN mutant G175C Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
G175C catalytically inactive mutant Xanthomonas campestris
additional information construction of a hrcN deletion mutant Xanthomonas campestris

General Stability

General Stability Organism
stability of HrcN depends on the conserved HrcL protein, which interacts with HrcN in vitro and in vivo Xanthomonas campestris

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane stability of HrcN in depends on the conserved HrcL protein, which interacts with HrcN in vitro and in vivo, both HrcN and HrcL bind to the inner membrane protein HrcU and specifically localize to the bacterial membranes under T3S-permissive conditions, overview Xanthomonas campestris 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Xanthomonas campestris The membrane-spanning type III secretion, T3S, system injects effector proteins into the cytosol of eukaryotic host cells, the T3S apparatus is associated with an ATPase that presumably provides the energy for the secretion process. HrcN is crucial for effector protein translocation and is essential for T3S and bacterial pathogenicity ADP + phosphate
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 ?
additional information Xanthomonas campestris stability of HrcN depends on the conserved HrcL protein, which interacts with HrcN in vitro and in vivo, overexpression of HrcL affects bacterial pathogenicity. HrcN also interacts with the T3S substrate specificity switch protein HpaC and the global T3S chaperone HpaB, which promotes secretion of multiple effector proteins, protein-protein interaction studies, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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 pathovar vesicatoria, a plant pathogen
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Purification (Commentary)

Purification (Comment) Organism
recombinant Strep-tagged wild-type and mutant enzymes by streptavidin affinity chromatography Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O The membrane-spanning type III secretion, T3S, system injects effector proteins into the cytosol of eukaryotic host cells, the T3S apparatus is associated with an ATPase that presumably provides the energy for the secretion process. HrcN is crucial for effector protein translocation and is essential for T3S and bacterial pathogenicity Xanthomonas campestris ADP + phosphate
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 ?
ATP + H2O HrcN hydrolyzes ATP in vitro Xanthomonas campestris ADP + phosphate
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 ?
additional information stability of HrcN depends on the conserved HrcL protein, which interacts with HrcN in vitro and in vivo, overexpression of HrcL affects bacterial pathogenicity. HrcN also interacts with the T3S substrate specificity switch protein HpaC and the global T3S chaperone HpaB, which promotes secretion of multiple effector proteins, protein-protein interaction studies, overview Xanthomonas campestris ?
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 ?
additional information HrcN dissociates a complex between HpaB and the effector protein XopF1 in an ATP-dependent manner. The effector release depends on a conserved glycine residue in the HrcN phosphate-binding loop, which is crucial for enzymatic activity and protein function during membrane-spanning type III secretion, T3S Xanthomonas campestris ?
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 ?

Synonyms

Synonyms Comment Organism
HrcN
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 Xanthomonas campestris
type III secretion ATPase
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 Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Xanthomonas campestris