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Literature summary for 7.4.2.8 extracted from
- Structural analysis of a prototypical ATPase from the type III secretion system (2007), Nat. Struct. Mol. Biol., 14, 131-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET28a expression vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN of Escherichia coli is presented at a resolution of 1.8 A | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| EscNDELTA102 | EscN mutant | Escherichia coli |
| EscNDELTA102-V393P | mutant, used for crystallization | Escherichia coli |
| EscNDELTA7 | EscN mutant | Escherichia coli |
| EscNDELTA7-R366D | EscN mutant | Escherichia coli |
| EscNDELTA8 | EscN mutant | Escherichia coli |
| G182C | EscN mutant | Escherichia coli |
| K183E | EscN mutant | Escherichia coli |
| R207G | EscN mutant | Escherichia coli |
| R209H | EscN mutant | Escherichia coli |
| R240H | EscN mutant | Escherichia coli |
| R366D | EscN mutant | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged EscN is purified from the soluble fraction using nickel-chelating Sepharose, the tag is cleaved with thrombin, cleaved product is purified by Mono-Q anion exchange | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EscN | - |
Escherichia coli |
| T3SS ATPase | - |
Escherichia coli |
| type III secretion system ATPase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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