Cloned (Comment) | Organism |
---|---|
using a pET29a based plasmid and pET15b for overexpressing of the protein in Escherichia coli BL21DE3 pLysS cells | Xanthomonas campestris |
Protein Variants | Comment | Organism |
---|---|---|
R286A | XpsE mutant hydrolysis ATP at a rate five times that of the wild-type XpsE, but is non-functional in protein secretion via T2SS | Xanthomonas campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0726 | - |
ATP | XpsE mutant R286A | Xanthomonas campestris | |
0.0891 | - |
ATP | XpsE wild-type, full-lenght | Xanthomonas campestris | |
0.1021 | - |
ATP | XpsE, N-domain removed | Xanthomonas campestris | |
0.1061 | - |
ATP | XpsE wild-type + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
0.1198 | - |
ATP | XpsE mutant R286A, N-domain removed | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Xanthomonas campestris | - |
ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
XpsE-Strep and its variants are purified from the Strep-Tactin Sepharose column, the XpsE with His6-tag is purified by eluting from nickel nitrilotriacetate resin | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Xanthomonas campestris | ADP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
T2SS ATPase | - |
Xanthomonas campestris |
type II secretion system ATPase | - |
Xanthomonas campestris |
XpsE | - |
Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
ATPase assay | Xanthomonas campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00062 | - |
ATP | XpsE wild-type, full-lenght | Xanthomonas campestris | |
0.00275 | - |
ATP | XpsE mutant R286A | Xanthomonas campestris | |
0.00305 | - |
ATP | XpsE, N-domain removed | Xanthomonas campestris | |
0.0036 | - |
ATP | XpsE mutant R286A, N-domain removed | Xanthomonas campestris | |
0.005 | - |
ATP | XpsE mutant R286A + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
0.00505 | - |
ATP | XpsE wild-type + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
0.00525 | - |
ATP | XpsE, N-domain removed + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
0.00558 | - |
ATP | XpsE mutant R286A, N-domain removed + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris |