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Literature summary for 7.4.2.8 extracted from

  • Shiue, S.; Chien, I.; Chan, N.; Leu, W.; Hu, N.
    Mutation of a key residue in the type II secretion system ATPase uncouples ATP hydrolysis from protein translocation (2007), Mol. Microbiol., 65, 401-412.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
using a pET29a based plasmid and pET15b for overexpressing of the protein in Escherichia coli BL21DE3 pLysS cells Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
R286A XpsE mutant hydrolysis ATP at a rate five times that of the wild-type XpsE, but is non-functional in protein secretion via T2SS Xanthomonas campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0726
-
ATP XpsE mutant R286A Xanthomonas campestris
0.0891
-
ATP XpsE wild-type, full-lenght Xanthomonas campestris
0.1021
-
ATP XpsE, N-domain removed Xanthomonas campestris
0.1061
-
ATP XpsE wild-type + MBP-tagged XpsL, residues 1-215 Xanthomonas campestris
0.1198
-
ATP XpsE mutant R286A, N-domain removed Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Xanthomonas campestris
-
ADP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
XpsE-Strep and its variants are purified from the Strep-Tactin Sepharose column, the XpsE with His6-tag is purified by eluting from nickel nitrilotriacetate resin Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Xanthomonas campestris ADP + phosphate
-
ir

Synonyms

Synonyms Comment Organism
T2SS ATPase
-
Xanthomonas campestris
type II secretion system ATPase
-
Xanthomonas campestris
XpsE
-
Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
ATPase assay Xanthomonas campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00062
-
ATP XpsE wild-type, full-lenght Xanthomonas campestris
0.00275
-
ATP XpsE mutant R286A Xanthomonas campestris
0.00305
-
ATP XpsE, N-domain removed Xanthomonas campestris
0.0036
-
ATP XpsE mutant R286A, N-domain removed Xanthomonas campestris
0.005
-
ATP XpsE mutant R286A + MBP-tagged XpsL, residues 1-215 Xanthomonas campestris
0.00505
-
ATP XpsE wild-type + MBP-tagged XpsL, residues 1-215 Xanthomonas campestris
0.00525
-
ATP XpsE, N-domain removed + MBP-tagged XpsL, residues 1-215 Xanthomonas campestris
0.00558
-
ATP XpsE mutant R286A, N-domain removed + MBP-tagged XpsL, residues 1-215 Xanthomonas campestris